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26 Cards in this Set
- Front
- Back
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Brom cresol purple |
MIO Purple = ornithine decarboxylated |
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Brom thymol blue |
OF glucose tubes Green = neutral Blue = protein Yellow = carb Open tube = oxidized Closed & open = fermented |
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Iodine |
Starch hydrolysis Dark = not digested. No amylase. Light = starch digested |
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Kovacs |
Add to SIM & MIO Red lake = indole present
Trypt—tryptophanase—> indole |
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Litmus |
Purple = neutral Blue = protein (caesin) Pink = fermented carb (lactose) |
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Methyl red |
Add to MR VP
Red MR = lactic acid Red VP = acetone |
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Nitrate A&B |
Add AB Red=reducer. Nitrite present.
Yellow = no nitrite. —> add zinc
Red = zinc reduced, not bacteria. Yellow = super reducer! |
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Phenol red |
Glucose lactose & sucrose fermentation tubes
Red = neutral Pink = alkaline. Protein Yellow = acidic. Carbs fermented. |
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Gram stain |
Crystal violet Iodine 95% alcohol Safranin
Purple = (+) Red/pink = (-) |
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Simple stain |
Heat fix
Crystal violet or carbol fuschion |
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Negative stain |
Nigrosin = anion |
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Acid fast |
Mycobacterium (mycolic acid in cell wall)
Carbol fuschion Acid Methylene blue
Red=acid fast Blue = not acid fast |
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Endosperm stain |
Malachite green Steam Safranin |
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Blood agar |
Beta = clear Alpha = cloudy Gamma = no zones |
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EMB |
Eosin methylene blue
Selects gm (-) Metallic green = fermented lactose |
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MacConkey’s |
Selects gm (-) Pink = fermented lactose |
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Mannitol salt |
Selects facultative halophes —streptococcus
Yellow = fermented mannitol |
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Mueller Hinton agar |
Abx testing |
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TNM |
Trypticase nitrate broth |
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PEA |
Phenyl ethanol agar Selects gm (+) —Staph & strep |
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R2A |
Melted for streak plate agar |
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SIM |
Sulfide (H2S) Indole Motility |
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SMA |
Standard methods agar |
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Thyoglycolate deeps |
Keeps O2 away Reducing agent Anaerobes vs. aerobes |
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YA10SC |
Loaded with sucrose. Encourages growth of streptococcus |
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Centrimide |
Selects pseudomonas |
