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16 Cards in this Set
- Front
- Back
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Mannitol Salt Agar Pink: basic, eats amino acids= S. Epi Red: Neutral -no growth Yellow: Acidic, ferments mannitol= S. Aureus |
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Eosin Methylene Blue Green: ferments lactose, Acidic= high coliform -> E.coli Purple/pink: ferments, Acidic -> coliform No color: ferments sacrose, non-coliform (usually pathogenic) |
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Pink: ferments lactose, Acidic (Ecoli) Yellow: Basic/Neutral ( salmonella) allow gram neg growth and inhibit gram+ |
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Thioglycollate
outcomes: 1.Obligate aerobe: must have oxygen 2.Obligate anearobe: harmed by oxygen 3. Facultative anerobe: do better with oxygen but can survive without 4. Microaerophile: require reduced level of oxygen 5. Aerotolerant: don't need oxygen but can survive if present |
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Oxidation – Fermentation
Green: neutral, no ferment or oxidation yellow: acidic, oxidation and fermentation Blue: Basic, no fermentation |
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Phenol Red Broth
Gas production: bubble in tube Yellow: fermentation, ? deaminase (because of fermentation) Red(control): No Fermentation (so no gas production), and no deamanse Pink (orangish): no fermentation, yas deaminase (check derm tube for gas) |
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Methyl Red TEST
Red-Positive Yellow- Negative |
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Voges Proskauer Test
Red:positive yellow/grey-green: negative |
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Catalase Test |
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Decarboxylation Test
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Kligler Iron Test |
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Motility Test
Top of Stab: Motile: - Obligate AreoTop Diffuse: Motile + Obligate Areo Diffuse Stab only: Motile: - |
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DNase Agar
Halo:postive No Halo: negative for DNase |
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Movable gelatin: positive Solid: negative |