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151 Cards in this Set

  • Front
  • Back

What should always be the primary consideration of the veterinary technician?

The animal's well-being.

The automation or mechanistic philosophical view on animals, supported by Rene Descartes, states that animals are ________ that ____ __ ____.

machines; feel no pain

The religious or dominion philosophical view on animals says that animals were put here by ___ and are not _______ _____.

God; morally equal

The utilitarian philosophical view on animals says that they feel pain and that the _______ of use must outweigh the _________.

benefit; suffering

SCAW supports the idea that ______ _________ is complementary to ____ _______.


What does SCAW stand for?

humane treatment; good science


Scientists Center for Animal Welfare

UFAW tries to approach animal welfare from the animal's _____ __ ____, with the goal being to simulate the animal's ________ and _____.


What does UFAW stand for?

point of view; behavior; needs


Universities Federation of Animal Welfare

What federation holds to the three R's proposed by Russel and Burch?

The Universities Federation of Animal Welfare (UFAW).

What are the three R's proposed by Russel and Burch?

Reduction, replacement, and refinement.

This group is classified by the FBI as a terrorist group.

Animal Liberation Front (ALF).

What is the twenty-eight hour law of 1873?

It stated that livestock being transported to market must be rested and watered at least once ever 28 hours during their journey. First federal law protecting animals against abusive treatment.


Who signed into law the AWA?

Lyndon B Johnson

Who decides which animals will be regulated under the AWA?

The Secretary of Agriculture

What genus of mice, and genus of rats, are excluded under the AWA?

Mice: Mus


Rats: Rattus

All research facilities undergo unannounced inspections by personnel from ______________.

Regulatory Enforcement Animal Care (REAC)

Class A dealers __________________, whereas Class B dealers _______________.

raise their own animals; buy and resell animals

What is an IACUC and what does it do?

Institutional Animal Care andUse Committee. Oversees regulatory compliance at an animal institution

An IACUC must include at least what 3 roles?

1) DVM (preferably ACLAM)


2) Participating scientist


3) Public member

ILAR first published this book in 1963.

The Guide for the Care and Use of Laboratory Animals.

What is the Controlled Substance Act?

Governs the use of any drug that people have the potential to abuse.

What is the Biologic Hazards Act?

Governs the control of humanpathogens, such as ebola, rabies, anthrax, etc.


What is the Lacey Act?

Governs the import, export, andinterstate commerce of foreign wildlife. Restricts importation of live specimens of harmful species.


What is the Endangered Species Act?

Provides the conservation ofendangered and threatened species of fish, wildlife, and plants.


What is the Marine Mammal Protection Act?

Requires that all marinemammals, and parts thereof, be imported, taken, possessed, purchased,sold, and/or transported under permit.


What is the major goal of a facility's macroenvironment?

To attain a "shell"

Why is it important to maintain the right room temperature for lab animals?

A temperature too high or too low can induce stress.

What is the ideal temperature range for mice, rats, hamsters, gerbils, and guinea pigs?

20-26 degrees C (68-79 degrees F)

What is the ideal temperature range for rabbits?

16-22 degrees C (61-72 degrees F)

What is the ideal temperature range for cats, dogs, and nonhuman primates?

18-29 degrees C (64-84 degrees F)

What is the ideal temperature range for farm animals and poultry?

16-27 degrees C (61-81 degrees F)

Rats get ________ at too low humidity.

ring tail

What is the ideal humidity of a lab animal facility?

30-70% relative humidity; 45-55% is best

How many changes of air per hour are required for a lab animal facility?

10 to 15

What are some examples of positive pressure rooms?

Surgical suites, barrier rooms, animal rooms

What are some examples of negative pressure rooms?

Animal quarantine, biohazard room, infectious animal rooms

Bright lights can damage the retinas of ______ rats and mice.

albino

____ can be put in wall blocks to reduce noise.

Sand

What are the environmental variables of a lab animal facility?

Temperature, humidity, ventilation, lighting, and noise

What are the support areas of a lab animal facility?


  • Hallways
  • Quarantine
  • Isolation
  • Cage Wash
  • Feed/Bedding
  • Equipment Storage
  • Administrative
  • Procedural

Quarantine is for __________ animals, whereas isolation is for ______ animals.

newly arrived; sick

___________ of new animals is done in quarantine.

conditioning

What are the animal room requirements of a lab animal facility?

Room surfaces, floors, door, and drains

The materials of the walls and ceiling of an animal room should be able to stand ____________ water that is ______.

high-pressure; heated

What kind of ceiling should be avoided in an animal room?

False ceilings

Floors must be properly sloped toward the ______.

drains

Doors should be covered with a __________________, whether ______________ or tough _______, to extend their life expectancy.

protective device; stainless steel; plastic

True or false: animal rooms should have hollow doors.

False

How large should the drains of an animal room be?

6 inches in diameter or larger

What is required of a conventional lab animal facility design?

No special requirements except controlling the environmental variables, and no provisions for "clean/dirty" traffic flow.

Clean/dirty lab setups allow personnel to better maintain _______ traffic flow. They lead to less _____________. There are 2 doors: _____ and _____.

one-way; contamination; clean; dirty

What are the cage design factors?

Comfort, safety, security, sanitation, functionality, accessibility, visibility, regulations.

How tall does a lab animal cage have to be?

Tall enough for the animal to stand in a "comfortable position" and change positions.

How do you calculate the amount of cage space a lab animal needs?

1) Measure animal length in inches (from tip of nose to base of tail), then add 6 inches

2) Square it

3) Divide by 144

Gives you amount in square inches, divide by 12 to get square feet.

When selecting the mesh size for the bottom of a cage, what is important to consider?

The size of the animal's feet

What kinds of animals are adept at escaping cages?

Non-human primates

Cage material must be able to withstand (3 things).

hot water, abrasive chemicals, and steam

This material tends to be the best material for cages, but it is also the most expensive.

stainless steel

Why is functionality important in cage design?

The animal may be used for specific research projects, and the cage should be the right kind for that project.

Why is accessibility important in cage design?

The animal care tech should be able to easily access the animal, and the cage must facilitate nutrition.

Cages that don't allow for easy visibility may (2 things).

- cause important data to be missed


- lead to potential health problems

Where can you find regulations for animal caging?

The Animal Welfare Act and The ILAR Guide for the Care and Use of Laboratory Animals.

What are some drawbacks of using galvanized steel cages?

It's very heavy, can't withstand modern cleaners and sanitizers, and they sometimes have a zinc coating which can be toxic to animals if they chew on the cage.

What are some advantages of using aluminum cages?

They are lightweight, sanitize easily, and are resistant to corrosion.

Stainless steel is the preferred metal for most lab animals, except rodents. Why don't you want to use it for rodents?

It conducts too much heat away from them, and makes them too cold.

How long do stainless steel cages last?

At least ten years (if maintained properly).

Polycarbonate is the most commonly used plastic for lab animal cages, and can stand up to a number of cleaning and sterilization regimens, but it is not recommended for ___________.

autoclaving

Polypropylene (what SCCC uses) has high heat and impact resistance, and is frequently found in use in __________________.

breeding colonies

Which plastic is clear to light amber in color, can be autoclaved up to 300 degrees F for 30 minutes, and is used for biohazards?

polysulfone

Which plastic is very low cost, making it good for disposable cages, and can be machine-washed at temperatures lower than 180 degrees F?

polystyrene

Which plastic is least used and highly resistant to chemical?

polyethylene

What is the standard cage shape for small rodents just being housed?

shoebox

What is a suspended cage used for?

Research studies in which the Principal Investigator wants to check body excretions.

What is a metabolism cage?

A specialized cage used for collecting urine and feces of research animals.

What is a restraining cage?

A device used to immobilize the animal for a procedure.

Which cage is highly utilized for non-human primates?

squeeze cage

What are transport cages usually made from?

cardboard

What do pens/activity cages allow an animal to do?

Exercise and interact with enrichment devices.

What are isolation chambers?

Specialized cages that hold animals to facilitate their use for exposure to test materials that are inhaled.

What kinds of cages are used for immunocompromised animals?

microisolators

What accessories and equipment might you need (depending on the animal) for a lab animal cage?

Cage lid, bowls, J-feeders, buckets, water bottles, automatic watering devices

What factors should be considered when selecting bedding for a lab animal cage?

Availability, absorbency, toxicity, that it's non-nutritive, comfort, disposability, and cost.

How many mice are used annually in lab research?

10.5 million

How are strains of lab mice identified?

The producer or company that breeds them.

What is the genus and species of the lab mouse?

Mus musculus

What is the class, order, and family of mice and rats?

Class -Mammalia


Order - Rodentia


Family - Muridae

Do male or female mice have spleens 50% larger than the other sex?

Males

What is the dental formula for mice, rats, and gerbils?

2 (1/1, 0/0, 0/0, 3/3)

Where is the breast tissue of a female mouse located?

Extends from the ventral midline over the flanks, thorax, and portions of the neck

Normal TPR of a mouse?

T - 37.1 C (98.8 F)


P - 427-697 BPM


R - 91-216/min

What is the normal food and water consumption of a mouse?

Food - 15g/100g/day


Water - 15g/100g/day

Do male or female mice fight?

Male

How much space do mice need in their cages?

At least 97 sq cm (15 sq inches) of floor space per adult.

What are some uses of laboratory rats?

Aging


Drugs (effects and toxicity)


Cavities


Vitamin effects


Bheavior


Alcoholism/drug effects


Arthritis


Infectious diseases

Why are rats better candidates for anatomical research than mice?

they're bigger

What are the most common strains of rats used in labs?

Sprague-Dawley (albino), Wistar (albino), and Long-Evans (hooded).

What organ do rats lack?

Gall bladder

What substance makes a rat's tears appear red, and where does it come from?

Porphyrin from the Harderian gland

Normal TPR for a rat?

T - 37.7°C (99.9°F)


P – 313-493 BPM


R – 71-146/min

What is the normal food and water consumption of a rat?

Food - 10g/100g/day


Water - 10mL/100g/day

When might female rats fight amongst each other?

When they're post-parturient.

How much cage space do rats need?

40 square inches of floor space per adult, and the cage should be at least 7 inches in height

What is the class, order, and family of the gerbil and the hamster?

Class: Mammalia


Order: Rodentia


Family: Cricetidae

Gerbils have unique physiological traits that make them good models for studying what conditions?

Epilepsy, infectious diseases, parasitology, water conservation, and lipid metabolism

What species has a distinct ventral abdominal pad composed of large sebaceous glands?

Gerbil

Are higher PCVs, hemoglobin levels, and total leukocyte counts found in male or female gerbils?

Male

What species might have some erythrocytes with prominent polychromasia and basophilic stippling?

Gerbils

Normal TPR for a gerbil?

T – 38.2°C (100.8°F)


P - 260-600 BPM


R - 85-160/min


What is the normal food and water consumption of a gerbil?

Food: 5g/100g/day


Water: 5mL/100g/day

What species is too curious for behavioral studies?

gerbils

What two species will usually return to their cage if they escape?

Gerbils and rats

Do male or female gerbils fight more?

Neither. They usually live peacefully in mixed groups of either sex if they're raised together

Young gerbils are rarely cannibalized, except (3 factors)

- excessive handling of young (by people)


- small litters


- lack of concealment

How often can a gerbil's bedding be changed?

Once a week

How often should a mouse's bedding be changed?

At least twice a week

How often should a rat's bedding be changed?

Once or twice a week

Gerbils can contract Tyzzer's disease, which is caused by (etiological agent).

Clostridium piliforme

Where did hamsters originate?

The Middle East

What kinds of studies are hamsters used for?

Dental research (caries)


Protozoal infections


Histocompatibility


Hypothermia and cancer research


Infectious diseases


Physiological studies

What color varieties of hamster are there?

Wild/reddish brown, cinnamon, cream, white, piebald

What is unique about a hamster's cheek pouches?

They have a high immunologic tolerance to homografts and heterografts.

Males of which species have prominent flank glands?

Hamsters

Which species has a forestomach similar to that of ruminants?

Hamsters

What is the normal TPR of a hamster?

T – 37.6 C (99.7 F)


P – 410-470 BPM


R – 38 – 110/min


What is the normal food and water consumption of a hamster?

Food - 15g/100g/day


Water - 10mL/100g/day

Which species pseudohibernates?

Hamsters

Pregnancy in a hamster can be detected by the absence of ___________________ at 5-9 days.

post-mating discharge

Why shouldn't you disturb a hamster and her litter for at least 7 days postpartum?

She is more likely to cannibalize the whole litter.

How much cage floor space do hamsters generally need? What is the exception?

19 square inches per animal


Breeding females need 150 square inches

What disease in humans has been associated with contact to hamsters?

Lymphocytic choriomeningitis (LCM)

What are two conditions hamsters are prone to?

Wet tail and dermatitis

What is the class, order, and family of the guinea pig?

Class: Mammalia


Order: Rodentia


Family: Cavidae

What kinds of studies are guinea pigs used for?

Human infections


Immunology


Genetics


Otology


Nutrition


Gnotobiology

What is the preferred strain of guinea pigs for pet and laboratory usage?

English

Which species has a palatal ostium?

Guinea pigs

How many nipples do guinea pigs have?

1 pair in the inguinal region

What is the dental formula of a guinea pig?

2(1/1, 0/0, 1/1, 3/3) = 20

The female of which species has a vaginal membrane that closes the orifice except during estrus and parturition?

Guinea pigs

Which species has a lower erythrocyte count, hemoglobin, and packed cell volume than most other rodents?

Guinea pigs

Normal TPR for a guinea pig?

T: 37.2 – 39.5 C (99-103.1 F)


P: 240-310 BPM


R: 42-104/minute


Normal food and water consumption of a guinea pig?

Food: 6g/100g/day


Water: 10mL/100g/day

Guinea pigs have a male-dominated hierarchy. What behavior do dominant males exhibit to other males?

Barbering

What is estrus characterized by in the guinea pig?

Lordosis reflex

How often should a guinea pig cage be changed?

Weekly

In which species is stampeding and erratic behavior common if the animal gets frightened?

Guinea pigs

In which species can antibiotic toxicity occur?

Guinea pigs

How many breeds and varieties of rabbits exist?

45 breeds, 77 varieties

What is the most common breed of rabbit used in lab research?

New Zealand White

How many pairs of nipples do female rabbits have?

4-5

What is the dental formula of a rabbit?

2(2/1, 0/0, 3/2, 3/3) = 28

Females of which species have two cervixes and no uterine body?

Rabbits

Normal TPR of a rabbit?

T: 38.5-40 C (101.3-104 F)


P: 180-250 BPM


R: 30-60/min


Normal food and water consumption of a rabbit?

Food Consumption: 5g/100g/day


Water Consumption: 5-15mL/100g/day