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Is a negative stain heat fixed?
NO
Negative Stain
Does not stain the bacteria but stains the background.
Nigrosin
Collodial stains are used in the negative stain technique.
When are negative stains useful?
Situations where other staining techniques don't clearly indicate cell morphology or size.
What are negative stains especially useful for?
Coccobacilli
Coccabacilli
short, oval bacilli or spirilla bacteria that are difficult to stain.