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34 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Lab 19 Discuss the meaning of selective toxicity in terms of antimicrobial chemotherapy. |
Selective toxicity refers to a chemicals ability to inhibit or kill only the microbe and not the host. |
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Lab 19 State why antimicrobial susceptibility testing is often essential in choosing the proper chemotherapeutic agent to use in treating an infection. |
Because there is no way to predict what drugs will be successful in a given case, espcially with emergence of antibiotic-resistant bacterium. |
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Lab 19 Interpret the results of a Bauer-Kirby antimicrobial susceptibility test when given a Mueller-Hinton agar plate, a metric ruler, and a standardized zone-size interpretation table. |
dip your head in a vat of jello and yell "Walla-Walla" |
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Lab 20 Define the following: protozoan, trophozoite, cyst. |
a) unicellular, eukaryotic microbes from Kingdom Protista. b) vegetative form of protozoans. c) protective form of some protozoans. |
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Lab 20 What causes recurring fever in malaria ? |
Lysis of infected red blood cells that release merozoites
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Lysis of infected red blood cells that release merozoites
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Lab 20 Recognize the following organisms when seen through a microscope and state what disease they are associated with: a. Entamoeba histolytica cysts in a fecal smear b. Trypanosoma gambiense in a blood smear c. Giardia intestinalis cysts in a fecal smear d. Trichomonas vaginalis in vaginal discharge e. Balantidium coli in a fecal smear f. Plasmodium merozoites in infected red blood cells |
A) amoebic dysentery B) African sleeping sickness. C) giardiasis D) genitourinary trichoniasis. E) balantidiasis. F)malaria |
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Lab 20 Define the following: a) helminth, b) ova, c) hermaphroditic. |
a) worms b) eggs? (not in text) c)contain both male and female sexual organs |
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Lab 20 List the three classes of parasitic helminths and state the common name for each class. |
Nematodes (Roundworms), Cestodes (Tapeworms), Trematodes (Flukes) |
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Lab 19 Define the following: a) antibiotic, b) antimicrobial chemotherapeutic chemical, c) narrow spectrum antibiotic, d) broad spectrum antibiotic. |
a) substances produced as metabolic products of one microorganism against another. b) synthesized chemicals which can be used therapeutically on microbes. c) effective against just gram + or - or only a few species. d) effective against a variety of gram+ and - microbes. |
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Lab 20 State how the following diseases may be transmitted to humans and briefly discuss how the diseases are diagnosed in the clinical laboratory: a. amoebic dysentery b. African sleeping sickness c. giardiasis d. genitourinary trichomoniasis e. balantidiasis f. malaria g. toxoplasmosis |
a. fecal-oral route. Diagnosed by fecal smear. b. bite from infected Tsetse fly. Observe blood, spinal fluid, or lymph node fluid for parasite. c. fecal-oral route. Cysts in fecal smear. d. venereal contact. Observe vagial discharge and urine under microscope. e. fecal-oral route. Fecal smear. f. Anopheles mosquito. Look for infected red blood cells. g. ingesting raw infected meat or inhailing cytes from cat feces. Serologic test or cell culture of organism. |
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Lab 22
State how the following diseases may be transmitted to humans and state how each disease may be diagnosed in the clinical laboratory: a. ascariasis b. pinworms c. trichinosis d. tapeworms e. flukes |
a. Fecal oral route. Fecal smear.
b. fecal-oral route or inhalation of ova. Applying tape to perianal region to see if ova are present. c. consuming poorly cooked pork containing the larvae. Serolocigal tests and biopsy. d. eating poorly cooked meat containing cysticerci. Observing feces for proglottids and ova. e. ingesting poorly cooked fish, crayfish, crabs, water vegetables. Looking for ova in urine, feces, or sputum? (not in text) |
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Lab 20 Recognize the following organisms or structures when seen through a microscope: a. roundworms b. Ascaris ova c. pinworm ova d. Trichinella in muscle tissue e. tapeworms f. scolex of a tapeworm g. ova and uterus in a gravid proglottid of a tapeworm h. flukes (recomend studying pictures and illustrations stop lots of fun stop) |
a. Elongated, unsegmented, cyndrilical. b. serrated edge c. smooth edge d. cyst in tissue containing larvae e. Flat segmented worms f. head structure with suckers and possibly hooks. g. section from the worm with a uterus full of eggs. h. unsegmented, flat, leaf-shaped. worms. |
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Define sterilization, disinfection, disinfectant and antiseptic |
A) destroying everything (free of all life forms) B) elimination of microorganism a except endospores C) disinfect objects but to toxic for human tissue. D) kills/inhibits microbes and is safe for human tissue |
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Lab 19 2 modes of disinfectants, antiseptics and sanitizer ( how they harm microorganisms) |
1) destroy cell membrane ( leakage of cellular material). 2) denature cells proteins ( blocks metabolism ) |
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Lab 19. Vitro test |
Artificial conditions |
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Lab 19 . Vivo? |
Actual conditions |
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Lab 19 . Resident flora ? |
Normal flora of skin |
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Lab 19. Transient flora ? |
Organisms you pick up from what you been handling |
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How do you remove flora ? |
Wash hands before /after |
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Lab 19 . Selective toxicity |
Chemical ability to inhibit/kill only the microbe not the host |
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Entamoeba histolytica picture |
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2 nucleus |
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Trapanosoma gambiense |
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Giardia lambila |
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two eyes look back at you |
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Balantidium coli |
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Blue with comma shaped thing inside |
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Trichomonas vaginalis |
Back (Definition)
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Plasmodium |
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Roundworms |
Back (Definition)
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Ascaris ova (picture) |
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Pinworm ova (picture) Enterrobius vermicularis |
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Trichinella in muscle tissue |
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Scolex of Tapeworm( Taenia pisiformis) |
Back (Definition)
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Tapeworm |
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Ova and uterus in Gravid proglottid of tapeworm |
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Flukes |
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Looks like a vag |
