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9 Cards in this Set

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What is essential when transferring bacteria to a sterile broth, slant, or agar plate is to maintain sterility by using ____

Aseptic Techniques

Why are Aseptic Techniques important?

To prevent contamination in the culture. This can confound your results in the identification of bacterium.

Common Aseptic Tools

1. Inoculating Loop & needle


2. Sterile transfer pipette


3. Glass spreading stick

One of the most important techniques in Microbiology

Streaking for isolation

What is the goal for isolation

toobtain aPURE bacterialculture (onlyone bacterial species)from aMIXED culture thatcontains2 or more bacterial species

Ubiquity

existing or being everywhere

Pathogenic Microorganisms

Disease Causing

Opportunistic Pathogens

•Normally inhabit our bodies withoutcausing disease


•These microorganisms cancause diseaseif introduced intoanew areaof the bodyor if theimmune system is suppressed (i.e., if the conditions are right themicrobes can seize the opportunity and cause disease)

Free-Living Microorganisms

•These microorganisms aren’t associatedwith any specific plant,or animal


•Non-pathogenic (notdisease causing)