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20 Cards in this Set
- Front
- Back
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What are cultures |
Used to grow microbes |
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What’s are sub cultures |
A culture made fro taking microbes from another culture |
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What is nutrient meida |
A substance containing food that is used for the growth of microbes that is usually added to culture broth |
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What is microbial isolation |
The production of single isolated colony of microbes |
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What Is inoculation |
The transferring of a sample in or onto an appropriate agar |
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What’s incubation |
Setting the best temperature and gas content for microbes to grow |
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Why do we need to isolate colonies |
-to allow as to inspect the colony colour and perform staining and smearing fro examination -to get pure cultures -to find the cell concentration in a sample -to allow us to do biochemical test to identify its species |
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What are techniques that can be used for isolation |
-streak plate -spread plate -pour plate - |
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How does spread plate work |
Adds the liquid sample onto agar and is spreaded by a spreader and is then incubated |
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How does pour plate work |
Sample is added to a sterile plate which liquid agar is then pour over it and then incubated to get a pure culture and CNA be used for cell enumeration |
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What are the incubation conditions for psychorphiles |
-10 or -20 degrees |
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What are the incubation conditions for human pathogenic bacteria |
37 degrees |
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What are the incubation conditions for obligate aerobes |
High amounts of oxygen |
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What are the incubation conditions for capnophiles |
High amounts of CO 2 |
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What are the incubation conditions for phototrophs |
Lots of sunlight |
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What isolations methods are suitable for obligate aerobes |
Streak and spread plate |
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What isolations methods are suitable for facultative anaerobes |
Spread streak and pour plate |
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What isolations methods are suitable for Obligate anaerobe |
Just pour plate |
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What isolations methods are suitable for microaerophiles |
Just pour plate |
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What isolations methods are suitable for aero tolerant anaerobe |
Pour streak and spread plate |
