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26 Cards in this Set
- Front
- Back
- 3rd side (hint)
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ISE |
Ion-selective electrode
Prevalence |
Routine methodology |
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ISE Routine methodology for measuring ______ in the clinical lab. |
electrolytes |
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ISE
Operation |
Insert two dissimilar metals (such as zinc or copper) into a conducting solution
called an “electrolyte”. One metal has a higher electron affinity than the other,
so a potential difference develops, producing electromotive force (EMF) or
voltage. Measure the potential difference (volts) between test electrode and
reference electrode. ISE uses ion selective probes, which respond to presence of a specific ion. |
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Nernst Equation |
ECell=EoCell-(0.0591/n)xlog(Q) |
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Nernst equation
_____=EoCell-(0.0591/n)xlog(Q) |
Ecell
What is it? |
TOTAL CHANGE IN VOLTAGE
based on change in voltage occurring from the sample ion conc. |
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Nernst equation ECell=______-(0.0591/n)xlog(Q) |
EoCell
What is it? |
Potential difference |
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EoCell
What is it? |
Potential difference defined by? |
Half cell rxns |
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ECell=EoCell-(0.0591/n)xlog(__) |
Q
What is it? |
Ratio Test ion solution Internal filling solution |
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ECell=EoCell-(0.0591/__)xlog(Q) |
n
What is it? |
charge of the ion |
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Half cell
Components |
Anode Cathode |
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Half cell
_______ Cathode |
Anode
Oxidized or reduced
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Oxidized |
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Half cell
Anode _______ |
Cathode
Oxidized or reduced? Why? |
Reduced Receives the released e- |
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Half Cell
Direction |
Anode moves toward the cathode |
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Half cell
Anode moves toward the _____ |
cathode
characteristics |
More + reduction potential |
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Ion-selective
Use of the unique properties of certain _____ materials to develop an _______________ for the measurement of specific ____ in solution. |
Use of the unique properties of certain membrane materials to develop an electrical potential for the ______ of specific ions in ________. |
measurement solution |
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Ion-selective
Selective membrane for a particular ion is in contact with? |
Both Test solution Internal filling solution |
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Ion selective
selective membrane for a particular ion is in contact with both test solution and ______________ |
Internal filling solution
What does it contain? |
Test ion at fixed conc. |
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Ion selective
What is the most common selective membrane for potassium measurement? |
Valinomycin is the most common selective membrane for _________ measurement? |
potassium |
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pH electrode
pH meters use pH probes, which are sensitive to? |
H+ ions |
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pH electrode
What develops between patient sample and buffer solution? |
Electric potential develops between _____ and _____ |
Pt. sample Buffer solution |
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pH electrode Electric potential develops between Pt. sample and buffer solution as _____ from the patient's sample diffuses through the ____ membrane. |
pH electrode
Electric potential develops between Pt. sample and buffer solution as H+ ions from the patient's sample diffuses through the glass _____. |
membrane |
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pH electrode
Electric potential develops between Pt. sample and buffer solution as H+ ions from the patient's sample diffuses through glass membrane.
This potential is measured by comparing to __________. |
Reference electrode
Equation |
E=Eo+(0.0591)*Log (H+ inside)/Outside |
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pH electrode
Since the inside (H+) is held ______ (reference), we can determine the H+ outside. |
Since the ______ (H+) is held constant (______), we can determine the H+ _____. |
inside reference outside |
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pH electrode
Since the inside ____ is held constant, we can determine ____ |
Since the inside H+ is held constant, we can determine H+ outside
THerefore, E is directly proportional to _________ |
Log (H+) of the sample |
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pH electrode
Since the inside H+ is held constant, we can determine H+ outside.
Therefore, E is directly proportional to _________ |
E is ___________ to Log (H+) of the sample |
directly proportional |
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pH electrode
___ is directly proportional to Log (H+) of the sample |
E is directly proportional to _____________ |
Log (H+) of the sample |
