Histopath Midterm

Front 1

SLIDING

Back 1

A type of microtome used on extremely hard, rough tissue blocks.

Front 2

CARBON DIOXIDE: -150 C

Back 2

Which of the following is incorrectly matched?

Front 3

INCLINATION

Back 3

Angle that refers to the side of the knife presenting to the block and surface of the block

Front 4

10-15 UM

Back 4

Standard thickness of sections in freezing microtome

Front 5

ADAMS

Back 5

SLIDING

Front 6

0.5 UM

Back 6

Usual thickness of an electron microscopy section

Front 7

25 MM

Back 7

Size of a plane wedge knife

Front 8

10-12 UM

Back 8

Usual thickness of sections produced by rocking microtome

Front 9

INCUBATOR

Back 9

A drying method that can be used on delicate tissues like nerves

Front 10

BOTH ARE TRUE

Back 10

Frozen tissue crumbs when the freezing procedure is not adequate. The block should be re-frozen before sectioning again.

Front 11

ONLY SECOND IS TRUE

Back 11

When the knife edgy is dirty, sections are lifted from the knife on upstrokes. This issue is also seen if the knife is dull

Front 12

MINERAL OIL

Back 12

Which of the following may be applied in the back of the stropping leather?

Front 13

ADJUSTMENT SCREW

Back 13

Regulates the thickness or the thinness of the sections produced while cutting

Front 14

INCUBATOR

Back 14

Drying method that can be used on sensitive or nervous tissues.

Front 15

BOTH ARE TRUE

Back 15

Too thin sections will make the nuclear stain too blue. Thick sections will make the cytoplasm pale

Front 16

ONLY SECOND IS TRUE

Back 16

When the knife edgy is dirty, sections are lifted from the knife on upstrokes. This issue is also seen if the knife is dull.

Front 17

SLIDING

Back 17

The knife and block holder moves at the same time in opposite directions

Front 18

BOTH ARE TRUE

Back 18

Incomplete f i xation leads to having milky clearing agent during clearing process. This can be resolved by repeating the entire process from fixation.

Front 19

BOTH ARE FALSE

Back 19

When the knife edge is covered with para?n, sections produced appeared to be squashed. This can also be observed when the clamp set screw on the knife or blockholder is loose.

Front 20

ONLY FIRST STATEMENT IS TRUE

Back 20

Rotary microtome is the ideal microtome for para?n embedded sections. It is stable and produce sections that are approximately 7-9 um thick.

Front 21

BOTH ARE FALSE

Back 21

When paraffin is impure, sections fail to form ribbons. This can be resolved by re-adjusting and re-orienting the block.

Front 22

l BOTH ARE FALSE

Back 22

Excessive dehydration tends to make the sections wrinkled. This can also occur when the paraffin too soft.

Front 23

BOTH ARE TRUE

Back 23

When the knife is tilted too much, sections fail to form ribbons. This can be resolved by re-adjusting and re-orienting the block.

Front 24

BOTH ARE TRUE

Back 24

Furrows in the sections are seen when the knife edge vibrates due to tissue hardnes. Colloidionizing the tissue will address this problem.

Front 25

BOTH ARE TRUE

Back 25

static electricity can cause the sections to adhere to the other parts of the microtome. Increasing the humidity and breathing out on the tissue gently would resolve this issue.

Front 26

BOTH ARE TRUE

Back 26

ooved and deformed sections can caused by sectioning in a warm room.Wrinkled sections can be caused by unparallel block edges.

Front 27

ONLY SECOND IS TRUE

Back 27

Holes in the section are formed if the knife is incorrectly sharpened. It can also be caused by hard spots in the tissue.

Front 28

BOTH ARE FALSE

Back 28

Split ribbons can be seen when the tilt on the knife is too small. This can be resolved by increasing tilt.

Front 29

BOTH ARE TRUE

Back 29

Sections adhere to the knife if there is low humidity. Increase the humidity by gently blowing on the tissue block.

Front 30

ROCKING MICROTOME

Back 30

Blocks are difficult to re-orient

Front 31

ULTRATHIN MICROTOME

Back 31

Tissues are f i xed using osmium tetroxide

Front 32

FREEZING MICROTOME

Back 32

Uses undehydrated tissue samples

Front 33

SLIDING MICROTOME

Back 33

Used for extremely hard, rough tissue blocks

Front 34

SLIDING MICROTOME

Back 34

Movable exposed knife

Front 35

CRYOSTAT/FREEZING

Back 35

used for cutting sections for silver staining demonstration

Front 36

ROCKING

Back 36

Ideal microtome for cutting paraffin sections

Front 37

ROTARY

Back 37

This microtome for operated by the use of flywheel

Front 38

ULTRATHIN OR SLIDING-BASE SLEDGE

Back 38

May be used for resin-embedded tissues

Front 39

SLIDING- STANDARD

Back 39

This requires a slow, steady motion