Hematology Review

Front 1

3 Stages of ESR

Back 1

1. rouleaux
2. settling
3. packing

Front 2

What is a Barr body, and where is it found?

Back 2

X chromosome precipitate
Neutrophil

Front 3

Describe what happens in the lag phase of the O2 dissociation curve

Back 3

Hgb is not able to fully load until one O2 binds

Front 4

Nucleus: cytoplasm ration in gran line

Back 4

myeloblast- 6:1
promyelocyte- 4:1
myelocyte- 1.5-1
meta- 1:1
Band- 1:2
seg -1:3

Front 5

Characteristics of myeloblast

Back 5

round nucleus and prominant nucleoli

Front 6

Lymphopoiesis

Back 6

lymphoblast
lymphocyte
T cell, B Cell (resting lymph)
activated lymph
Plasma cell

Front 7

Granulocyte maturation

Back 7

myeloblast
promyelocyte
myelocyte
metamyelocyte
band
seg

Front 8

Degrees of detection and what found in flow cytometry

Back 8

0- size
10- complexity
90- lobularity and granularity

Front 9

Plots %O2 vs. hbg o2 saturation

Back 9

o2 dissociation curve

Front 10

Way to determine Hgb []

Back 10

whole blood -> solution with KCN, KFeCN-> oxidizes heme mlcl.

color intensity measured at 540nm.

Front 11

Drabkins reagent

Back 11

KCN & KFeCN

used in hemoglobin determination

Front 12

Causes methemoglobin from hemoglobin

needed for cyanmethemoglobin

Back 12

ferricyanide

cyanide

Front 13

Hematocrit determined from____ by

process

Back 13

hgb 3X rule

whole blood in microcapillary-> centrifuge, measure %cells:volume

Front 14

Retic stain

Back 14

Supravital stain

Front 15

Ways to deal with difficult plasma in Hgb measurements

Back 15

plasma replacement

Front 16

Codocyte aka_

Back 16

target cell aka____

Front 17

Acanthocyte vs. echinocyte

Back 17

irregular and significant vs. poor drying/staining and insignificant (regular)

Front 18

Drepanocytes

Back 18

Sickle Cells

Front 19

increased lipids, bilirubin, WBC (looks like)

Hgb S or C causes

Back 19

creamy, yellow, turbid plasma (caused by)

hard to lyse cells

Front 20

Basophilic stippling aka

Back 20

punctate basophilia aka

Front 21

Papenheimer bodies- erythrocyte

Back 21

smaller than Howell jolly bodies.

clumps of Fe (multiple)

Front 22

Howell Jolly bodies- erythrocyte

Back 22

DNA remnants- spleen failure

Front 23

Induced DIC (activates FX)

Back 23

Russell's viper venom

Front 24

Fibrinogen testing

Back 24

reagent- thrombin

shows how much is left (little= DIC/eclampsia)

Front 25

Factor assay theory

Back 25

deficient pt. plasma added to plasma with adhesion of one factor. If no clot= missing pt. factor

Front 26

Siderosome

Back 26

Papernheimer body- iron

Front 27

Fibrin split products & D-Dimer testing is

Back 27

quantitative (yes or no) determine break down of clots

Front 28

total blood let from George

Back 28

3.75 L

Front 29

Reagent for PT

Back 29

thromboplastin and Ca++ (innovin)

Front 30

APTT looks at_____

PT looks at _______

Back 30

intrinsic efficiency test

extrinsic efficiency test

Front 31

Why use elegic acid?

Back 31

XII -> XIIa
negative charge

Front 32

ISI

Back 32

international sensitivity index

Ideal @ 1

Front 33

INR calculation

Back 33

R^ ISI

Front 34

INR based on

Back 34

reagents used, method/ instrument, sensitivity

Front 35

R (for INR calculation) is

Back 35

pt protime/ mean normal population protime

Front 36

Von behrens transducer

Back 36

avoids impedence with plate

Front 37

Test for sickle cell

Back 37

osmotic fagility
sickle dex

Front 38

Platelet estimates

Back 38

# plts in 30 fields (by tens), average 2 closest (20 fields).

Divide instrument given # by counted #. calibration factor.

Front 39

plt. maturation

Back 39

Megakaryoblast
promegakaryocyte
megakaryocyte
mature plt.

Front 40

PT reagents

Back 40

tissue thromboplastin (checks prothrombin)

Front 41

exudates vs. transudates

Back 41

exudates- infected
transudates- lots of fluid (non infection)

Front 42

What do you dilute cloudy or bloody fluid with?

Back 42

cloudy (WBC)- glacialacetic acid
RBC- saline

Front 43

Butt cells

Back 43

lymphoma characteristic cell

Front 44

keys for neutrophilic toxicity

Back 44

toxic granules
left shift
vacuoles
Dohle bodies

Front 45

Coincidence loss correction

Back 45

2 cells through at the same time/ repeated (cycling) of cells

Front 46

Volumetric metering

Back 46

mercury tells volume

Front 47

Wright stain aka______

Back 47

Romanowsky stain aka _______

Front 48

Wright stain dyes

Back 48

polychromatic dye
eosin
methaline blue

Front 49

Erythrocyte Line

Back 49

pronormal blast
basophilic normoblast
polychromatic normoblast
orthochromic normoblst
polychromatic erythrocyte (retic)
RBC

Front 50

last erythrocyte maturation cell to have nucleus

Back 50

orthochromic normoblast