Hematology Benchwork Part 1

Front 1

Skin puncture sites for older children and adults

Back 1

Palomar surface of the non-dominant hand, 3rd and 4th finger

Front 2

Recommended depth of skin puncture for children

Back 2

<2mm

Front 3

Possible effects of squeezing on skin puncture

Back 3

Hemolysis

Excess interstitial fluid

Front 4

Why discard 1st drop of blood

Back 4

Discard excess tissue fluid, dead epidermal cells and facilitate free flow of blood

Front 5

Order of draw for skin puncture

Back 5

Tube for blood gas analysis

Slides

EDTA

Other anticoagulant microtubes

Serum microtube

Hint 5

TSEOS

Front 6

Cleanse the site using friction scrub with movement____

Back 6

Back and forth

Front 7

Most common sites for venipuncture

Back 7

Superficial veins of the anticubital fossa

Front 8

Angle between skin and needle

Back 8

<30

Front 9

Tourniquet application time

Back 9

<1 minute

Front 10

Effects of prolonged tourniquet application

Back 10

Hemolysis

Hemoconcentration

Shortened coagulation time

Front 11

False shortening of clot based coagulation test is a result from

Back 11

Prolonged tourniquet application

Front 12

What factors accumulate in prolonged tourniquet application

Back 12

Factor VIII and VWF

Front 13

Distance of tourniquet from venipuncture site

Back 13

3-4 inches above

Front 14

Most common needle size for adult venipuncture

Back 14

21G

Front 15

Pumping of fist during venipuncture will increase?

Back 15

Potassium

Front 16

Most common cause of needle puncture

Back 16

Improper disposal

Front 17

Optimal anticoagulant concentration for EDTA

Back 17

1.5 unit/ml of blood

Front 18

Action of EDTA

Back 18

Chelation of calcium

Front 19

Blood spx for CBC must be analysed within _____ of collection

Back 19

6 hours

Front 20

Blood smears should be made within __ hours of collection

Back 20

3 hours

Front 21

Effect of over filled EDTA tube

Back 21

Presence of clots

Front 22

Effects of underfilled EDTA tube

Back 22

Decrease HCT, ESR

Increase MHC, PLT

Front 23

Anticoagulant of choice for OFT

Back 23

Heparin

Front 24

Most widely used anticoagulant for plasma and whole blood chemistry test

Back 24

Lithium Heparin

Front 25

# of inversions for sodium Citrate

Back 25

3-4

Front 26

Ratio of anticoagulant and blood in sodium citrate

Back 26

1:9

Front 27

Actions of SPS anticoagulant

Back 27

Prevent phagocytosis

Prevent complement activation

Neutralizes antibiotics

Front 28

Tan tap is for

Back 28

Lead determination

Front 29

Royal blue top is for

Back 29

Toxicology

Nutritional chem

Trace elements

Front 30

White top is for

Back 30

Molecular biology

Front 31

Pink top is for

Back 31

Blood bank test

Front 32

Disadvantage of EDTA blood smear

Back 32

Platelet satellitosis

Front 33

Correction for platelet satellitosis

Back 33

Re-collect specimen with 3.2% Sodium Citrate

Front 34

Factor to be used to correct platelet count satellitosis

Back 34

1.1

(PLT conc X 1.1)

Front 35

Most frequently used method of blood film preparation

Back 35

Two-glass slide method (manual wedge technique)

Front 36

Angle between 2 slides on manual wedge technique

Back 36

30-40 angle

Front 37

Too high angle will make smear

Back 37

Thicker

Front 38

Size of the drop of blood for smear

Back 38

2-3mm

Front 39

If increased HCT, angle for smear should be___

Back 39

Lower

Front 40

Ideal blood smear characteristics

Back 40

Gradual transition from thick to thin area

2/3 to 3/4 length of the film side

Finger shape

Front 41

The only advantage of coverslip method for smear

Back 41

Excellent WBC distribution

Front 42

Problem on stain:

Rbc: gray

WBC: too dark

Eosinophil granules: gray

Back 42

Stain/buffer is too basic

Inadequate rinsing

HEPARINIZED blood was used

Front 43

Problem in stain:

Rbc: too pale or are red

WBC: barely visible

Back 43

Stain/buffer too acidic

Underbuffering

Overbuffering

Front 44

Probable reason for blood smear bluer than normal

Back 44

Patient has increased protein

Front 45

Probable reason for holes all over the smear

Back 45

Patient has increased lipid levels

Front 46

If snowplow effect and fibrin strands is observe on blood film, medtech should?

Back 46

Reject sample

Front 47

Part of blood film where rbc appear as macrocytic, flattened and lack central pallor

Back 47

Feathered edge

Front 48

Part of blood film where rbc appear as microcytic and may appear as rouleaux

Back 48

Thick part