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30 Cards in this Set
- Front
- Back
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Myeloperoxidase stain (MPO)
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Mature Neutrophils and Eos pos
Monos slightly pos Lymphs neg |
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Cyanide-resistant peroxidase
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Eos positive
Others neg |
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Acid Phosphatase
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T-cells pos
B-cells neg |
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Tartrate-resistant Acid Phosphatase (TRAP)
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Hairy-cells positive
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Leukocyte Alkaline Phosphatase (LAP)
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Only neutrophils pos
Score indicates maturity |
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Periodic-Acid-Schiff
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Neutrophils strong/Monos weak pos
Lymphs/erythroblasts neg Stains glycogen pink |
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Sudan Black B
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Granulocytes pos
Lymphs Neg Stains phospholipids/lipoproteins blue |
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Non-specific Esterase (NSE)
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Monos pos
(alpha-napthyl esterase) stains black |
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Naphthol AS-D chloroacetate esterase
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Neutrophils pos
Basos weak pos/neg Lymphs/Monos/Eos neg |
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Prussian Blue
+siderocyte formula |
Precipitates free iron into granules
%siderocytes=#RBCs pos/100 RBCs Reference: 0-1% |
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Chediak Hegashi Syndrome
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Betaglucocerebrosidase deficiency
large lysosome granules/dohle bodies/partial albinism |
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Gaucher's Disease
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lipid storage disease
(large monocytes) |
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Tay-Sachs Disease
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hexosaminidase A def.
(monos) |
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Niemann-Pick
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sphingomyelinase def.
(monos) |
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CD4
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T-Helper
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CD8
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T-suppressor
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ALL - FAB L1
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pre-B cells
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ALL - FAB L2
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Adult T-cell
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ALL - L3
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Burkitt's lymphoma - leukemia phase (B-cells)
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CLL
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B-cells
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Hairy-cell Leukemia
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B-cells
TRAP Positive |
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Waldenstrom's Macroglobulinemia
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Plasma (B) Cell malignancy
M-protein |
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Hodgkin's Lymphoma
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Reid-Sternberg cells
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AML - M1
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90% Myeloblasts
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AML - M2
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<90% myeloblasts w/Auer Rods
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AML - M3
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Promyelocytic
Auer Rods |
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AML - M4
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AMML 30 myeloblasts in marrow
M4e - w/eosinophilia |
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AMoL - M5
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Monocytic
DIC and renal insuff. |
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CMML
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Myelodysplastic
<30% Blasts in periph. Alpha-napthyl esterase pos |
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AEL - M6
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Di Guglielmo's disease
PAS Positive |
