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13 Cards in this Set
- Front
- Back
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STAINS |
simply means coloring the microorganisms with a dye that emphasizes certain structure |
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POSITIVE STAINING |
involves a positive stain, in which the dye actually sticks to the cell and gives them color. |
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NEGATIVE STAINING |
the dye does not stick to the specimen but settles around its outer boundary, forming a silhouette. |
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these are the dyes most commonly used for negative staining |
NIGROSIN (BLUE-BLACK) AND INDIA INK ( BLACK ) |
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TYPES OF POSITIVE STAINING |
-simple (requires only 1 dye) -differential (two different color dyes) -special ( used to color and isolate specific parts of microorganisms such as flagella, reavel the presence of capsules) |
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The mostly wide employed staining in bacteriology |
GRAM STAINING |
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It uses a crystal violet as stain, iodine as mordant, acetone/alcohol as decolorizer and safranin red as counter stain. 1 MINUTE -1 MINUTE- 10/30 sec- 30 sec |
GRAM STAINING |
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ACID FAST (takes up the most primary stain (PINK) NON-ACID FAST- take up the secondary stain (BLUE) |
ZIEHL-NEELSEN METHOD |
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Carbon Fucshin-Primary Stain- 5 minutes ( heat in hot plate) Acid Alcohol Wash - 15 seconds Methylene Blue- Secondary Stain-1 minute |
ZIEHL-NEELSEN METHOD |
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It is stained with malachite green. Heat is used to provide stain penetration. The rest of the cell is then decolorized and counterstained a light red with safranin. |
ENDOSPORE STAINING |
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the Crystal violet gives bacterial cells and capsular material dark purple dark. |
CAPSUOE STAININGA (ANTHONY'S CAPSULE STAINING METHOD) |
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CRYSTAL VIOLET - PRIMARY STAIN ( 4-7 mins) Copper Sulfate - Counter Stain |
CAPSULE STAINING |
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HISS METHOD |
1. Take clean or grease free slide 2. Add a drop of crystal violet 3.Prepare smear 4. Air dry the smear 5. Flood copper sulphate 6. Air dryband add of oil immersion 7. Microscopic observation |
