Gram Positive Bacilli

Front 1

Epidemiology of Listeria monocytogenes

Back 1

1. frequently found in environment
2. causes stillbirths and meningitis in animals, especially ruminants
3. in humans: seen in neonates, pregnant & immunocompromised
4. Assoc. w/ foodborne illnesses

Front 2

Listeria monocytogenes Identification characteristics

Back 2

FACULTATIVE anareobe
beta hemolysis on SBA
Catalase + / Esculin hydrolysis +
tumbling motility >25C
Cold enrichment - grows well @ 4C

Front 3

Epidemiology of Corynebacteria

Back 3

1. normal flora of humans and animals
2. N.F. of skin, oral cavity, genitourinary tract
3. opportunistic
4. considered a contaminant

Front 4

Corynebacteria shared ID characteristics

Back 4

Gram + bacilli - pleomorphic rods palisading or chinese characters
Most CATALASE +

Front 5

Corynebacterium diptheriae
identification

Back 5

Methylene blue: shows metachromatic granules
loeffler agar slant
SBA - rule out Strep A
Tellurite or Tinsdale - will turn black
Elek plate toxin test +

Front 6

Corynebacterium diptheriae
disease manifestation

Back 6

pharyngitis w/ thick leathery gray membrane
exotoxin when C. diptheriae infected with TOX + Phage
death assoc w/ asphyxiation & myocarditis

Front 7

Corynebacteriaum jeikeium (CDC JK group)
disease manifestation

Back 7

infection in immunocompromised
- Prosthetic heart valces
- Septicemia
- Skin infections

Front 8

Nocardia asteroides characteristics

Back 8

produce aerial hyphae
always a pathogen - pulmonary infections, brain abscesses
molar resembling colonies
GRAM + bacilli branching
partially ACID-FAST w/ modified Kinyoun stain

Front 9

Gardnerella vaginalis characteristics

Back 9

facultative anaerobe
Catalase -
GRAM VARIABLE pleomorphic rods
FASTIDIOUS
Bacterial vaginosis
-fishy odor w/ vaginal discharge in 10% KOH
-CLUE cells

Front 10

Erysipelothrix rhusoppathiae

Back 10

facultative anaerobe
Catalase -
Nonmotile
grows in 8.5% NaCl
good growth in SBA, CNA, Chocolate
produces H2S on Triple Sugar Fe
localized infection on skin, usually fingers and hands

Front 11

Lactobacillus general characteristics

Back 11

normal flora of lower GI & genital tract
long, slender Gram + rod
Vaginal infections assoc. w/ ABSENCE of lactobacilli

Front 12

Bacillus general characteristics

Back 12

low virulence saprophytes
common contaminant in lab
SPORE producing
Gram -, large, boxcar shaped rods may stain gram VARIABLE
Strict AEROBE
Catalase +

Front 13

Bacillus antracis characteristics

Back 13

2 types of manifestations in humans:
Pulmonary - breathe in spores, resembles cold first few days
Cutaneous

Front 14

Bacillus cereus

Back 14

food poisioning w/ rice & eye, wound infections

Front 15

Bacillus subtilis

Back 15

lab contaminant

Front 16

Clostridium general characteristics

Back 16

inhabit soil, water, vegetation, bowels
Gram + large rods (may be gram variable)
ANAEROBES
SPORE producing

Front 17

Clostridium perfringens
identification

Back 17

many exotoxins, type A most significant
large, box-car shape G+ bacilli
B-hemolytic on SBA (2x zone)
rapid growht
HIGH AMTS OF GAS
Lechthinase + on egg yolk agar

Front 18

C. perfringens infections

Back 18

Gas gangrene
Food poisoning