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66 Cards in this Set
- Front
- Back
What type of E.coli if found in GI tract and non pathogenic but can cause infection in compromised patients
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Commensal E.coli
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What type of E.coli are pathogenic group of diarrheal E.coli that cause infection in GI tract
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Diarrheagenic E.coli
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What type of pathogenic group cause extraintestinal infection?
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Extra intestinal E.coli
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Heat labile enterotoxins (LT) and heat stable toxin (St) belongs to which E.coli
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Entero toxigenice E.coli
ETEC |
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Form aggregative adherence on tissues/cells
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Entero aggregative E.coli
EAEC |
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Adhere to intestinal mucosa and cause extensive host cell actin rearrangement belong to which E.coli
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Entero pathogenic E.coli
EPEC |
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Primary cause of hemorrhagic colitis (HC) or bloody diarrhea --> HUS belong to which E.coli
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Entero Hemorrhagic E.coli
EHEC STEC |
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Which E.coli is similar to EPEC except it produce shiga toxins (Stx)
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EHEC hemorrhagic
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Which E.coli closely resemble shigella but do not form shiga toxinx (STX)
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EIEC
Entero invasive E.coli |
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Which E,coli cause invasive, dysenteric form of diarrhea
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EIEC
entero invasive E,coli |
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Which E.coli forms a diffuse adherent pattern to tissues/cells?
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Diffusely adherent E.coli
DAEC |
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Not associated with foodborne outbreak yet
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Diffusely adherent E.coli
DAEC |
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Which bacteria is GRAM -ve rod of enterobacteriaceae family
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E.coli O157 : H7
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Natural habitat of E,coli
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Intestinal tract of cattle
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Virulence factors of E.coli
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Shiga like toxins 1 2
Intimin Tir |
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Site of infection of E.coli O157 : h7
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Large intestine
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Mechanisms of E.coli bacteria
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Adherence: E.coli's intimin bind to Tir on the epithelial cells --> colonize --> AE lesion
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E.coli O157:h7 produce?
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Shiga like toxins Stx 1 and Stx 2
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How shiga toxins work ?
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Bind to receptor on epithelial cells
Once internalized, Stx toxins inhibit protein synthesis ---> host cell death ---> bloody diarrhea |
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Disease of E,coli O157 : h7
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Bloody diarrhea -----> hemorrhagic colitis
Kidney failure- HUS |
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Both E EC and E EC cause AE lesions and are invasive resulting in bloody diarrhea?
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EPEC EHEC
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EHEC is more virulent due the production of ?
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Shiga like Toxin (STX)
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E EC and E EC are not invasive but can produce enterotoxins which lead to watery diarrhea
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ETEC EAEC
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what 3 methods of detection of E.coli O157: H7
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1.biochemical / cultural methods
2.Immunological methods 3.Molecular methods |
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what color of colonies appear on sorbitol MacConkey agar for E.coli O157:H7
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White
other E.coli - pink (biochemical methods) |
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E.coli 0157 :H7 are lactose positive , sorbitol negative and beta glucuronidase negative
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Correct
E.coli O157 :H67 |
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O antigen code for
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Somatic /LPS
E.coli O157 :H67 |
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H antigen code for
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Flagella
E.coli O157 :H67 |
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Fecal.............of agricultural lands by domesticated and wild animals
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Adulteration
E.coli O157 :H67 |
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Gram -ve rods of Enterobacteriaceae family that do not ferment lactose
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Salmonella
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Important foodborne Salmonella serovars
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S.enterica serovar Typhimurium , Enteritidis, New port , stanley
Salmonella |
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Serovars of Salmonella based on ?
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O,H and K antigens
K antigen : capsule |
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How Salmonella spread?
3 methods |
Carriers
Animal Feeds Fecal/water |
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Enteristis (infection at small intestine)
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Salmonella
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Most frequent isolates
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S.enterica serovar Typhimurium
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what type of serovar bacteria found in poultry and eggs
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S.enterica serovar Enteritidis
Poultry and eggs |
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Salmonella are reclassified into which 2 species based on DNA
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S.bongori
S. enterica |
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4 important Salmonella serovars
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Typhimurium
Enteritidis Newport Stanley |
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Cell surface ruffling in which 2 bacteria?
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Salmonella Typhimurium and shigella spp
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How do cell surface ruffling work?
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inject virulence genes in host
Entry through ruffling Multiply and spread in host cells Cause apoptosis of host cells |
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WhAT are injected through type III secretion system (TTSS)
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Effectors
Salmonella |
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Bacteria that do cell ruffline
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Salmonella and shigella
E.coli NO ruffling, only assist |
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Which bacteria is Gram-ve rods non motile.
most strains are non - lactose fermentor |
Shigella
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Which are the 2 common species of Shigella
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S.sonnei
s. flexneri |
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Which bacteria can be foodborne and non foodborne
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Shigella
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What type of bacteria are involved in Food contaminated due to poor personal hygiene or fecal oral transmission
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shigella
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What type of bacteria are involved in food that are served
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shigella
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Which bacteria is the 3rd largest foodborne monitored by foodnet
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shigella
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How the pathogenesis of Shigella works?
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Invade epithelial cells of small or large intestine through ruffling
Multiply intra cellularly Spread from cell to cell by actin-- based motility like listeria |
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Which bacteria mediated cell ruffling and internalization?
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Salmonella
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Which bacteria mediated induction of apoptosis
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shigella
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Shigella mediated induction of apoptosis produce?
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Shiga toxins that are thermostable and inhibit protein synthesis.
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Other virulence factors of Shigella mediated induction of apoptosis?
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Apoptosis of macrophages
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Which gram- rods bacteria of enterobacteriacease family are motile at 25, but not 37?
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Yersinia Enterocolitica
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Common serotypes of Yersinia Enterocolitica
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O:3 and O:8
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which bacteria may involved in other illness such as reactive arthirtis (ReA) and thyroid disorders?
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Yersinia Enterocolitica
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Which bacteria is an pathogenic invasive m/o?
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Yersinia Enterocolitica
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how pathogenesis of Yersinia Enterocolitica works?
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YadA an adhesin that binds to host integrin on M cells in small intestine
Adhere and invade host cells Heat stable enterotoxin, YsT local and systemic inflammation |
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Which bacteris is gram negative spirally curved small rods: motile
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Campylobacter jejuni
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What are grow in our lumen which needed only 3-6% oxygen and 10% CO2
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microaerophilic
Campylobacter jejuni |
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Pathogenesis of Campylobacter jejuni
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May produce enterotoxins
recruit white blood cells/inflammation induce host cell death may lead to autoimmune disease |
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Virulence factor for Campylobacter jejuni
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Thermostable direct hemolysin TDH
Cholera toxin Adherence factors: fimbriae, hemagglutinins |
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Vibrio cholerae produces what kind of toxin in the lumen?
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Cholera Toxin (CT)
Campylobacter jejuni |
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How vibrio work?
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Cells attach to M cells by toxin co-regulated pili (TCP)
Produce CT which induces cAMP and results in water secretion in lumen. CT subunit A1 activates adenylate cyclase through ADP- ribosylation |
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What are the 4 type of pathogenesis of intracellular bacteria?
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1.Adherence/attachment
2.Entry/invasive 3.Growth and spreading 4.Toxin/ enzyme |
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which 4 types of Gram negative pathogens used Type III secretion system ( TTSS)
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1.E.coli O157:H7
2.Salmonalle 3.Shigella 3.yersinia |