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18 Cards in this Set

  • Front
  • Back
List two advantages of PCR (polymerase chain reaction) over cloning
faster, can use template that's been degraded
list two ways you can try to determine the function of a new discovered rat gene
1. compare to known that are already known
2. RNAi knowdown
List 2 mechanisms of gene regulation that are used by eukaryotes but not prokaryotes
1. RNAi
2. Alternative splicing
Genomics
study of overall genetic structures of species
probe
fragment of DNA that is labeled and hybridized to a target molecule
RFLP
fragments of DNA cut of restriction enzymes and compared between individuals
genetic drift
loss of alleles from a population due to sampling bias
What are the two basic requirements for the molecular study of a gene that can be met by either PCR amplification or cloning
1.look at tiny fragments of DNA
2. make lots of copies of that tiny fragment of DNA
List two advantages that cloning has over PCR (Polymerase chain reaction)
1. can copy larger fragments
2. more accurate
List two different causes of spontaneous small or large scale mutations
1. tautomerization
2. Base depurination
List two techniques that are used in functional genomics
1. microarrays
2. knockout mice
ultraviolet light
common cause of thymine dimers
genomic library
collection of many cloned DNA fragments from a single organisms
recombinant DNA
DNA combined from two different sources
trinucleotide repeats
common cause of DNA strand slippage
ionizing radiation
common cause of DNA strand breakage
List two different things that are known to induce small small mutations
1. UV light
2. chemicals
List two techniques used to screen many individuals to detect allele frequencies for population/evolutionary genetic studies
1. PCR of microsatellites
2. gel elecrophoresis