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18 Cards in this Set
- Front
- Back
List two advantages of PCR (polymerase chain reaction) over cloning
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faster, can use template that's been degraded
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list two ways you can try to determine the function of a new discovered rat gene
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1. compare to known that are already known
2. RNAi knowdown |
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List 2 mechanisms of gene regulation that are used by eukaryotes but not prokaryotes
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1. RNAi
2. Alternative splicing |
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Genomics
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study of overall genetic structures of species
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probe
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fragment of DNA that is labeled and hybridized to a target molecule
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RFLP
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fragments of DNA cut of restriction enzymes and compared between individuals
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genetic drift
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loss of alleles from a population due to sampling bias
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What are the two basic requirements for the molecular study of a gene that can be met by either PCR amplification or cloning
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1.look at tiny fragments of DNA
2. make lots of copies of that tiny fragment of DNA |
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List two advantages that cloning has over PCR (Polymerase chain reaction)
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1. can copy larger fragments
2. more accurate |
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List two different causes of spontaneous small or large scale mutations
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1. tautomerization
2. Base depurination |
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List two techniques that are used in functional genomics
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1. microarrays
2. knockout mice |
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ultraviolet light
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common cause of thymine dimers
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genomic library
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collection of many cloned DNA fragments from a single organisms
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recombinant DNA
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DNA combined from two different sources
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trinucleotide repeats
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common cause of DNA strand slippage
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ionizing radiation
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common cause of DNA strand breakage
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List two different things that are known to induce small small mutations
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1. UV light
2. chemicals |
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List two techniques used to screen many individuals to detect allele frequencies for population/evolutionary genetic studies
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1. PCR of microsatellites
2. gel elecrophoresis |