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25 Cards in this Set
- Front
- Back
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genetics is the study of...
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how genes vary and are passed down
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define gene
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a funcitonal unit on a chromosome
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what are three major categories of genetics
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molecular
transmission population |
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what is molecular genetics
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study of the nucleotide sequence. covers genomes, genomics, and bioinformatics
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what is transmission genetics
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pattern of inheritance of traits. covers breeding
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what is population genetics
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looks at gene frequencies and variation in entire populations. covers evolution
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define chromosome
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a unit of DNA that continas many genes that can be replicated
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describe genetic material
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carries traits
mechanism of trait expression variable mutable transmittable |
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what did hershey and chase do
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protein has sulfur
DNA has phosphorus marked with labels found that DNA is hereditary material |
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what did griffith do
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S heat + R = dead mouse
proves transformation |
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what did avery macleod and mccarty do
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used proteinase
ribonuclease dNase found that transformation cannot occur without DNA therefore DNA is hereditary material |
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what is the biologically significant form of DNA
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B
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is RNA stable?
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no
ss |
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is RNA base or acid labile
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base labile
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how many bounds between AT
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two
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what are three ways to manipulate DNA
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gradient centrifugation procedures
reassociation kinetics nucleic acid electrophoresis |
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how does gradient centrifugation work
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solution with gradient
sample separates by density expose to UV light works because DNA fluoresces in UV light and absorbs UVA260 |
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hybridization =
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renaturing
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does ssDNA absorb more or less light than ds
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more
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in dissociation kinetics, a sample that dissociates slowly must....
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be more CG rich
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define tm
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half the sample is melted
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define shearing
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breaking DNA into small pieces
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what does absorption of 1 mean? 0?
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1 is all ss
0 is ds |
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why is agarose gel used
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big pieces get stuck and separate
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A/T = C/G
true or false |
true 1 = 1
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