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36 Cards in this Set

  • Front
  • Back

Type IIIS

Living, virulent, sickly strain

Type IIR

Living, nonvirulent

His experiment led to the discovery that there was some sort of transforming principle that can be passed from dead bacteria to living to change their characteristics

Griffith

Discovered that DNA was the transforming principle

Avery

Has two OH groups on its pentose sugar

RNA

2 ringed structure

Purines

1 ring carbon structure

Pyrimidines

The purines

Adenine and Guanine

The pyrimidines

Cytosine, Uracil, and Thymine

The covalent bond in DNA or RNA that links the 3' carbon of the sugar of one nucleotide to the 5' phosphate group of the adjacent nucleotide

Phosphodiester bond

Removes the RNA primer and replaces it with DNA

DNA polymerase I

Prevents supercoiling

DNA gyrase

Separates the two DNA strands

DNA helicase

Bind to single strands of DNA and prevent re-annealing from occurring

SSB proteins

Bind to the OriC sequence

Initiator proteins

An RNA polymerase that synthesizes a short RNA primer to initiate DNA replication

Primase

Has 5' to 3' exonuclease function

DNA polymerase I

Main DNA synthesizer, elongation

DNA polymerase II

Used for splicing, removes introns

snRNA

Non-template DNA polarity

5' to 3'

Template DNA polarity

3' to 5'

mRNA strand polarity

5' to 3'

Where transcription begins

+1 region

Part of the DNA where the RNA separates the strands of the DNA and begins synthesis of RNA

-35 and -10 region

A sequence of nucleotides at the 5' end of the mRNA that does not encode any of the amino acids of a protein

5' UTR (leader)

Sequence of nucleotides at the 3' end of the mRNA not translated into protein

3' UTR (trailer)

An extra nucleotide at the 5' end of the mRNA and methyl groups on the base in the newly added nucleotide and on the 2'-OH group of the sugar of one or more nucleotides at the 5' end

5' cap

String of adenine nucleotides added to the 3' end of a eukaryotic mRNA after transcription

poly(A) tail

Sequence in bacterial DNA that requires the presence of the rho subunit of RNA polymerase to terminate transcription

Rho-dependent terminator

Sequence in bacterial DNA that does not require the presence of the rho subunit of RNA polymerase to terminate transcription

Rho-independent terminator

Subunit of bacterial RNA polymerase that allows the RNA polymerase to recognize a promoter and initiate transcription

Sigma factor

Promoter region for eukaryotic RNA polymerase II promoters, determines the starting point for transcription

TATA box

Polypeptide chain found in several different transcription factors that recognizes and binds sequences in eukaryotic promoters

TATA-binding protein

Synthesizes mRNA

RNA polymerase II

Required for high level expression and tissue specific expression

Enhancers

Synthesizes the poly A tail

Poly A polymerase