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22 Cards in this Set
- Front
- Back
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What features of DNA structure enable it to be replicated? |
1. The double stranded structure 2. Base pairing between A & T, G & C |
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The complementarity of DNA strands is based on |
the AT/GC rule |
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To make a new DNA strand, which of the following is necessary? |
A template strand Nucleotides |
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The model that correctly describes the process of DNA replication is |
the semiconservative model |
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What are the functions of the AT-rich region and DnaA boxes? |
It causes the AT- rich area to unwind. |
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How many replication forks are formed at the origin? |
Two replication forks are formed at the origin. |
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A site in a chromosome where DNA replication begins is |
an origin of replication |
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The origin of replication in E. coli contains |
an AT-rich region. DnaA box sequences. GATC methylation sites. |
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Why is primase needed for DNA replication? |
B/C DNA polymerase can not initiate DNA replication on a bare template strand. |
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Is the template strand read in the 5′ to 3′ or the 3′ to 5′ direction? |
3′ to 5′ direction |
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Is DNA strand breakage needed for catenane separation? |
Yes, so the strands can move relative to each and untangle the catenane. |
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The enzyme known as uses and separates the DNA strands at the replication fork. |
helicase, ATP |
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In the lagging strand, DNA is made in the direction the replication fork and is made as . |
away from, Okazaki fragments |
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Does the oxygen in the newly made ester bond come from the phosphate or from the sugar? |
The oxygen bond in the newly made ester bond comes from the sugar. |
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DNA polymerase III is a processive enzyme, which means that |
it does not dissociate from the growing strand after it has attached a nucleotide to the 3′ end. |
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The proofreading function of DNA polymerase involves the recognition of a and the removal of a short segment of DNA in the direction. |
base mismatch, 3′ to 5′
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Why do eukaryotes need multiple origins of replication? |
Because eukaryotes chromosomes are so large, they need multiple origins so the DNA can be replicated in a reasonable length of time. |
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How many times would telomerase have to bind to a different site in the telomere to make a segment of DNA that is 36 nucleotides in length? |
Six times |
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In eukaryotes, DNA replication is initiated at an origin of replication by |
the origin recognition complex. |
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Which of the following statements regarding DNA polymerases in eukaryotes is not correct? |
DNA polymerase α synthesizes a short RNA-DNA primer. DNA polymerases ε and δ synthesize most of the leading and lagging, respectively. Lesion-replicating DNA polymerases can replicate over damaged DNA. |
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In eukaryotes, RNA primers are primarily removed by |
flap endonuclease. |
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To synthesize DNA, what does telomerase use as a template? |
It uses RNA that is a component of telomerase. |
