Genetics 19

Front 1

Another name for recombinant DNA technology is

Back 1

genetic engineering.

Front 2

Short DNA sequences that are repeated in tandem and that are commonly used in DNA fingerprinting are called

Back 2

microsatellites

Front 3

Mutations can be induced at specific locations through which process?

Back 3

Site-directed mutagenesis

Front 4

Which type of restriction enzyme cuts the DNA within or near the recognition sequence?

Back 4

Type II

Front 5

Which of the following is a next-generation sequencing technique?

Back 5

Pyrosequencing

Front 6

_____ mice are genetically altered animals in which a particular DNA sequence has been added to a known chromosome location.

Back 6

Knockin

Front 7

Restriction enzymes occur naturally in

Back 7

bacteria

Front 8

A set of bacterial colonies or phages that contain all the DNA sequences found in a genome is called a(n)

Back 8

genomic library

Front 9

An organism that has been permanently altered by the addition of a foreign DNA sequence to its genome is said to be

Back 9

transgenic

Front 10

Gel electrophoresis is a technique for

Back 10

separating DNA fragments on the basis of their size

Front 11

Determining the particular order of bases in a DNA molecule is known as

Back 11

DNA sequencing.

Front 12

Which of the following is a concern that has been raised about genetic engineering of agricultural products?

Back 12

Negative effects of genetically engineered crops on biodiversity

Possible hybridization with native organisms

Potential negative effects of releasing novel organisms into the environment

The potential for ecological disruption

Front 13

A technique for quickly amplifying DNA fragments is

Back 13

polymerase chain reaction

Front 14

A technique in which neighboring genes are used to locate a gene of interest is called

Back 14

chromosome walking

Front 15

The transfer of DNA segments into humans to treat disease is known as

Back 15

gene therapy

Front 16

A DNA or RNA molecule that is complementary to a sequence of interest and that is often used to find the sequence of interest is a called a

Back 16

probe

Front 17

Short DNA sequences that are repeated in tandem and that are commonly used in DNA fingerprinting are called

Back 17

microsatellites

Front 18

_____ mice are genetically altered animals in which a normal gene has been fully disabled.

Back 18

Knockout

Front 19

Enzymes that make double-stranded cuts in DNA at specific nucleotide sequences are called _____ enzymes.

Back 19

restriction

Front 20

A set of bacterial colonies or phages that contain all the DNA sequences found in a genome is called a(n)

Back 20

genomic library

Front 21

_____ mice are genetically altered animals in which a particular DNA sequence has been added to a known chromosome location.

Back 21

Knockin

Front 22

Which is an important requirement for a cloning vector?

Back 22

Contains an origin of replication

Front 23

Determining the particular order of bases in a DNA molecule is known as

Back 23

DNA sequencing.

Front 24

An organism that has been permanently altered by the addition of a foreign DNA sequence to its genome is said to be

Back 24

transgenic

Front 25

Small DNA fragments that contain one or more restriction sites and that can be attached to the end of a DNA molecule are termed

Back 25

linkers

Front 26

Mutations can be induced at specific locations through which process?

Back 26

Site-directed mutagenesis

Front 27

A large plasmid that has been used to transfer genes into plant cells is the _____ plasmid.

Back 27

Ti

Front 28

The traditional approach to genetics that begins with a phenotype and proceeds to find the gene responsible for that phenotype is called _____ genetics.

Back 28

forward

Front 29

Which of the following is one of the challenges of working at the molecular level?

Back 29

Each cell contains thousands of genes.

Front 30

What is the purpose of reverse transcriptase in the creation of a cDNA library?

Back 30

It synthesizes DNA from mRNA.

Front 31

The transfer of DNA segments into humans to treat disease is known as

Back 31

gene therapy

Front 32

The approach to genetics that begins with a gene and proceeds to determine the phenotype caused by the mutated gene is called _____ genetics.

Back 32

reverse

Front 33

A technique for transferring fragments of DNA from a gel to a permanent solid medium is

Back 33

Southern blotting

Front 34

Determining the particular order of bases in a DNA molecule is known as

Back 34

DNA sequencing.

Front 35

For what purpose would one use a bacterial artificial chromosome?

Back 35

To clone a large DNA fragment (larger than 100,000 bp)

Front 36

Initial knockout mice are chimeras containing cells with the disabled gene and cells with the wild-type gene. In order to study the phenotype of mice lacking the gene of interest, what must be done?

Back 36

Intercross the chimeric mice, and some of the progeny will be homozygous for the defective gene.

Front 37

A scientist wants to perform site-directed mutagenesis, but the appropriate restriction enzyme
sequences are not available to mutate the target gene. What is a strategy he could employ?

Back 37

Oligonucleotide-directed mutagenesis

Front 38

An organism that has been permanently altered by the addition of a foreign DNA sequence to its genome is said to be

Back 38

transgenic

Front 39

The Sanger method of DNA sequencing uses a special substrate that causes DNA synthesis to terminate. This special substrate is a

Back 39

dideoxyribonucleotide

Front 40

A technique in which neighboring genes are used to locate a gene of interest is called

Back 40

chromosome walking

Front 41

Why is it important that a cloning vector have a unique site for each restriction enzyme?

Back 41

The presence of several restriction sites will generate multiple DNA fragments that cannot be easily reassembled.

Front 42

Which of the following best describes a cDNA library?

Back 42

Consists of DNA sequences that are expressed

Front 43

Genetically engineered plants with the Bt gene have which property?

Back 43

Produce an insecticidal toxin

Front 44

An organism that has been permanently altered by the addition of a foreign DNA sequence to its genome is said to be

Back 44

transgenic