Gel Electrophoresis

Front 1

Outline the process of Gel Electrophoresis

Back 1

Extracting the DNA
Separating the DNA
Visualising the DNA
Isolating the gene of interest

Front 2

Extracting the DNA

Back 2

DNA extracted

Front 3

Separating the DNA

Back 3

DNA cut by restriction enzymes placed in wells in polyacrylamide gel in buffer solution with electrodes at either end

DNA fragments travel along the gel to the ANODE or positively charged end according to size. DNA fragments thus separated

Front 4

Visualising the DNA

Back 4

Polyacrylamide gel contains ethidium bromide which unites with the DNA and fluoresces under UV Light
DNA placed in dark room and UV light shone on DNA so can see fragments

Front 5

Isolating the gene of interest

Back 5

A marker is loaded onto the gel
Details size of gene of interest
Marker ocmpared to fragments to find gene of interest

Front 6

Fate of the DNA after gel elecrtophoresis?

Back 6

The gene of interest could be removed from the gel, purified and then cloned