Fixation

Front 1

An example of an additive fixative is one that contains:

Back 1

Mercuric chloride

Front 2

When compared with tissue fixed in formalin, tissue fixed in zinc-formalin will show:

Back 2

superior nuclear detail

Front 3

Microscopic examination of H&E stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following:

Back 3

Underfixation

Front 4

Microscopic evaluation of H&E sections from a surgically removed small bowel specimen show an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from:

Back 4

Delayed fixation

Front 5

A specimen of kidney must be shipped to another city for immunofluorescence studies. The specimen shoud be placed in:

Back 5

Michel Solution

Front 6

In a certain project it is importan to use a fixative that contains acetic acid yet stabilizes erythrocyte membranes. One fixative that could be used is:

Back 6

Hollande Solution

Front 7

When a microwave oven is used for fixation, the most critical factor is the:

Back 7

control of the temperature

Front 8

To adequately remove the calcium in a specimen containing areas of microcalcification, the tissue could be fixed in:

Back 8

Hollande solution

Front 9

Which of the following fixatives contain copper acetate:
Hollande
Bouin
Gendre
Zamboni

Back 9

Hollande

Front 10

A specimen is submitted with the statement that it was fixed in formalin. Microscopic sections show marked lysis of erythrocytes. This indicates thate the fixative most likely was:

Back 10

acetified with acetic acid.

Front 11

Fixatives are classifed as additive because of the:

Back 11

additon, or binding of the fixative, to tissue proteins

Front 12

The only kidney biopsy tissue available has been fixed in phosphate-buffered glutaraldehyde for 2 hours and then placed in phosphate buffer solution. If a portion of this tissue is processed for light microscopy, sections would most likely show:

Back 12

non specific PAS staining

Front 13

Uric acid crystals are preserved only when tissue is fixed in:

Back 13

Absolute alcohol

Front 14

Improper preservation of tissue will result if there is:

Back 14

a delay in fixation

Front 15

A good fixative will:

Back 15

protect tissue against alteration by subsequent processing

Front 16

the function of methanol in commercial formalin soltuions is to:

Back 16

retard the polymerization of formaldehyde

Front 17

the subsequent demonstration f chromaffin granules requires which of the following fixatives?
Orth
Bouin
B-5
Formalin

Back 17

Orth

Front 18

In electron microscopy, Zamboni fluid, glutaraldehyde, and osmium tetroxide function as:

Back 18

fixatives

Front 19

Tissues will remain unfixed if placed in:

Back 19

Sodium borate

Front 20

Bouin solution is contraindicated for:

Back 20

tissue to be stained by the Feulgen reaction