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19 Cards in this Set
- Front
- Back
Animal Cell Culture requires: |
Appropriate medium to grow on (agar or broth) Appropriate temperature Correct osmotic balance Suitable pH Oxygen (if aerobic) Aseptic |
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Cell culturing |
Ability to grow very small organisms in the laboratory. Allows millions of cells to be grown in a small area, with hundreds of generations studied over a short time. Also allows mutations to be detected, isolated and studied |
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How? -Animal cell |
Cells are chemically or physically removed from a source
Cells placed in a vessel and incubated at optimum temperature
Animal cells are anchored to the vessel and nutrients, water, antibodies and growth factors are present to grow the cells |
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Nucleus |
Genetic information bearer of the cells Locations of chromosomes DNA replication and transcription occur |
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Nucleolus |
Contains genes for ribosomal RNA synthesis (rRNA) |
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Mitochondria |
ATP producer of the cell (energy) Involved in cellular respiration used to synthesize ATP |
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Chloroplast |
Glucose synthesis Absorb light to carry out photosynthesis |
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Ribosomes |
Protein synthesizes of the cell |
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Vacuole |
Storage containers of the cell Water, nutrients |
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Golgi apparatus |
Package proteins Post office of the cell |
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Rough ER |
Protein assembly line of the cell |
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Cytoplasm |
Thick jelly-like substance of the cell |
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Lysosome |
Enzyme-packed wrecking balls of the cell |
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Active transport |
LOW ➡ HIGH Needs energy (ATP) Uses protein pumps Against the concentration gradient
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Passive transport |
HIGH ➡ LOW no energy needed With the concentration gradient |
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Diffusion |
Movement of molecules from HIGH ➡ LOW concentration No energy needed With the concentration gradient Small molecules that move through phospholipids of the cell membrane (water, carbon dioxide) |
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Facilitated Diffusion |
HIGH ➡ LOW No energy needed With the concentration gradient Larger molecules that cannot fit through the phospholipids, so they move through a channel protein Charged ions |
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Osmosis |
HIGH ➡ LOW No energy needed With the concentration gradient Occurs through phospholipids Only water |
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Regulatory Proetins |
Regulate the cell at the checkpoints |