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4 Cards in this Set
- Front
- Back
Transcription |
DNA is transcribed to make RNA by means of RNA polymerase
Three phases: Initiation, Elongation, Termination
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Transcription Initiation |
-Promoters: non coding DNA located upstream of a gene -Sigma factor: helps RNA polymerase bind promoters; "holoenzyme" |
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Transcription Elongation |
-RNA Polymerase reads coding DNA strand -RNA Polymerase 'spits out' mRNA
*In prokaryotes, transcription and translation happen at the same time |
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Transcription Termination |
-Rho dependent: protein factor Rho destabilizes the interaction between the template and mRNA, newly synthesized mRNA is released from the elongation complex
-Rho independent: newly synthesized RNA molecule forms a G-C rich hairpin loop followed by a run of U's |