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18 Cards in this Set
- Front
- Back
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Water Dissociation Constant
Keq = |
Keq= [H][OH]/[H2O]
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pH=
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pH=-log[H]
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Bronstead Acid
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Releases a proton
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Bronstead Base
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Accepts a proton
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pKa=
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pKa=-log(Ka)
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Henderson-Hasselbalch Equation
pH = |
pH = pKa + log([A-]/[Ha])
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What is a polyprotic acid?
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An acid that has more than one acid group
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1st Law of Thermodynamics
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Energy can be neither created nor destroyed
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2nd Law of Thermodynamics
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Universe is moving towards a constant increase in entropy (disorder)
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Hess's Law
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For a particular set of reactants going to a particular set of products, the Enthalpy change is the same regardless of steps
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delta G^0 =
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delta G^0 = -RT ln(Keq)
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Keq =
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Keq = e^(-delta G/RT)
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Chromatography
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Stationary phase and a mobile phase
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Ion Exchange Chromatography (for anions)
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Protein in anionic (-)
Resin is cationinc (+) Protein binds to charged resin Resin is washed to remove contaminates. Protein is washed off with a stronger salt solution |
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Size Exclusion Chromatography
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Larger molecules pass through faster
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Affinity Chromatography
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Specific binding of a component of the mixture. Other molecules pass through. Bound molecules are washed off later.
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HPLC
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Very fine separation of components
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Electrophoresis
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Uses the charge of the sample mixture to separate components
Positively charge molecules travel towards the cathode (-) |
