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30 Cards in this Set
- Front
- Back
structure => inheritance
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nucleotide bases -> information
base complementarity -> replication |
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Griffith
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R -> S => xfm
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Avery et al.
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DNase -> -S
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Hershey-Chase
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phage 32P DNA -> cell
35S protein -> phage ghost |
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nucleotide
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phosphate + deoxyribose + base
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purine
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AG
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pyrimidine
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TC
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Chargaff's rules
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T + C = A + G
T = A C = G A + T ~= G + C |
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Franklin
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X-ray diffraction:
DNA long, skinny, parallel length-wise |
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backbone
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-- 5' - phosphodiester - 3' --
antiparallel |
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pairing
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1' - base---base - 1'
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stacking
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plate -> stability
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grooves
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major (most ptn assoc)
minor |
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replication models
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* semiconservative - P-D P-D
conservative - P-P D-D dispersive - PD-PD PD-PD |
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Meselson-Stahl
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15N P + 14N medium -> 14/15 D + 14/14 D'
CsCl gradient -> banding |
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replication fork
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Cairns: cold DNA + hot cell -> seam
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DNA polymerase
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base--3' O <- 5' P--complement
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pol III
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catalyzes synthesis at fork
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replication strands
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--- 3'---5' leading <-
--- 5'---3' lagging -> |
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Okazaki fragment
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short stretches at lagging strand
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lagging strand synthesis
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primase -> 5'--3' RNA primer
pol III 3'---> primer---> pol III ---------> ---> DNA ligase --------> |
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pol exonuclease activity
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proofreading
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replisome
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pol III holoenzyme + accessory ptns
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pol III holoenzyme
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leading + lagging catalytic cores
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beta clamp
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pol III distributive -> processing
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helicase
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base--/ /--base
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SSB proteins
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prevent helix reformation
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topoisomerase
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cut, rejoin to relax supercoils
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DnaA
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initiate replication:
recognize oriC bind to DnaA box melt ATs to unwind recruit replisome |
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helicase
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bind and slide 5' -> 3' to unzip helix
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