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14 Cards in this Set

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DNA Rep-1
What does semiconservative replication mean?
2 daughter double stranded DNA molecules each contain on strand of the parent DNA
DNA Rep-1
What 3 observations have been made for all DNA Polymerases?
1) Incoming bases are selected by complimentary base pairing by hydrogen bonding with a template strand.

2) Chain growth is in the 5'-3' direction and antiparallel to the direction of the template strand.

3) All DNA Polymerases require a primer strand to add bases to.
DNA Rep-1
What is the function of DNA Polymerase I?
Proofreading and editing capabilities
1) 5'-3' DNA Polymerase - Joins deoxynucleotides using a primer and a free 3' end.

2) 3'-5' Exonuclease - Remove mismatched bases from growing DNA strand.

3) 5'-3' Exonuclease - Snow plow effect removing any mismatched bases in its path. 10 bases at a time.
DNA Rep-1
What is the Klenow Fragment?
Larger part of the Dissociated DNA Polymerase 1.
Contains the polymerase and the 3'-5' exonuclease.
DNA Rep-1
What is the function of DNA Polymerase III?
Chief DNA replicating enzyme for E Coli.
Binds only to single stranded regions. 'high processivity' due t its sliding clmap feature which moves along the template strand
DNA Rep-1
What is the function of a topoisomerase?
Change the topography of DNA molecules by cutting strands, allowing the amount of supercoiling to be adjusted, and then religating the strands
DNA Rep-1
What is the function of a Helicase?
Catalyze the unwinding of double stranded DNA by disrupting the base-pair hydrogen bonding
DNA Rep-1
In replication, which strand is the leading strand, and which strand is the lagging strand?
Leading - Synthesized in the same direction of the DNA replication fork. Replication on the leading strand is continuous.

Lagging - Discontinuous replication in the opposite direction of the DNA replication fork.
DNA Rep-1
How is RNA used in DNA Replication?
Primer sequence for DNA replication to occur
DNA Rep-1
What are Okazaki fragments?
Primer sequences with short pieces of RNA attached to them
DNA Rep-1
Where is E Coli DNA replication initiated?
DnaA binding to oriC
DNA Rep-1
Where is E Coli DNA replication terminated?
Ter protein binding to TAU site
DNA Rep-1
What is the processivity of DNA Polymerase I?
20 base pairs
DNA Rep-1
What is the processivity of DNA Polymerase III?
Entire 5 million base genome of E Coli