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17 Cards in this Set

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Formation of biofilm.
Pellicle Formation, bacterial attachment, multiplication and colonization, maturation, and matrix formation
4 steps
Dental Biofilm
Dense, non mineralized, colonies in a gel like intermicrobial matrix.
Adheres to the acquired pellicle
May be supra, or sub gingival
Contains more then 500 species + yeast, protozoa, and viruses
Acquired pellicle
a tenacious membranous layer, forms after removal of external debris
Amorphous
A cellular
ORGANIC!!
Types of pellicles
Surface pellicle- unstained
Surface pellicle-stained
Subsurface pellicle- demineralized area
Hydroxyapatite
INORGANIC, mineralized
microscopic crystals forms on the pellicle
absorbs glycoproteins
Functions of the pellicle
Protective layer
Lubricant
Aids in bacteria adherence
Aids in calculus adherence
Changes in Biofilm Microorganisms
As biofilm matures, wbc increase, more gram - and anaerobic organisms; gingival inflammation - 7 to 14 days.
Older biofilm- vibrios & spirochetes leads to gingivitis - 14 to 21 days
Biofilm Composition
°INORGANIC Elements
-Calcium, Fluoride and Phosphorus are higher in concentration
°ORGANIC Elements
-Carbs, glutans and fructans that aid in adherence of bacteria
-Proteins, saliva and gingival exudate
-Lipids, endorphins from gram - bacteria.
Where do we find Biofilm?
Supra and Subgingival, fissures, proximal formation, GINGIVAL 3RD OF THE TOOTH, crowded teeth, rough areas, malocclusions, (difficult to clean areas)
Detection of Biofilm
Direct view - disclosing agent
Explorer or probe - pull off materia alba
Clinical records - location, index
Impact of Biofilm
Sets the stage for perio and caries
Foundation for calculus
Mal-odor
Dental Caries
Low fluoride content
Streptococci mutants and sorbrinis. Lactobacilli, activities and nonmutans strep are acidogenic.
Cariogenic foods, time, biofilm, pH and carbs.
pH in the mouth that causes decay
enamel 4.5 to 5.5
cementum 6 to 6.7
normal mouth 6.7 to 7.0
Main bacteria in Biofilm that causes perio?
Actinobacillus actinomycetemomitans.
Materia Alba
White material, loosely attached to plaque,
Soft like cottage cheese
Visible
Contributes to gingival inflammation and caries.
Removable
normal pH
6.5 to 7.0
what contributes to gingival inflammation
food debris, biofilm, calculus