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17 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Formation of biofilm.
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Pellicle Formation, bacterial attachment, multiplication and colonization, maturation, and matrix formation
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4 steps
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Dental Biofilm
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Dense, non mineralized, colonies in a gel like intermicrobial matrix.
Adheres to the acquired pellicle May be supra, or sub gingival Contains more then 500 species + yeast, protozoa, and viruses |
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Acquired pellicle
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a tenacious membranous layer, forms after removal of external debris
Amorphous A cellular ORGANIC!! |
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Types of pellicles
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Surface pellicle- unstained
Surface pellicle-stained Subsurface pellicle- demineralized area |
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Hydroxyapatite
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INORGANIC, mineralized
microscopic crystals forms on the pellicle absorbs glycoproteins |
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Functions of the pellicle
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Protective layer
Lubricant Aids in bacteria adherence Aids in calculus adherence |
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Changes in Biofilm Microorganisms
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As biofilm matures, wbc increase, more gram - and anaerobic organisms; gingival inflammation - 7 to 14 days.
Older biofilm- vibrios & spirochetes leads to gingivitis - 14 to 21 days |
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Biofilm Composition
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°INORGANIC Elements
-Calcium, Fluoride and Phosphorus are higher in concentration °ORGANIC Elements -Carbs, glutans and fructans that aid in adherence of bacteria -Proteins, saliva and gingival exudate -Lipids, endorphins from gram - bacteria. |
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Where do we find Biofilm?
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Supra and Subgingival, fissures, proximal formation, GINGIVAL 3RD OF THE TOOTH, crowded teeth, rough areas, malocclusions, (difficult to clean areas)
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Detection of Biofilm
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Direct view - disclosing agent
Explorer or probe - pull off materia alba Clinical records - location, index |
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Impact of Biofilm
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Sets the stage for perio and caries
Foundation for calculus Mal-odor |
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Dental Caries
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Low fluoride content
Streptococci mutants and sorbrinis. Lactobacilli, activities and nonmutans strep are acidogenic. Cariogenic foods, time, biofilm, pH and carbs. |
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pH in the mouth that causes decay
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enamel 4.5 to 5.5
cementum 6 to 6.7 normal mouth 6.7 to 7.0 |
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Main bacteria in Biofilm that causes perio?
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Actinobacillus actinomycetemomitans.
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Materia Alba
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White material, loosely attached to plaque,
Soft like cottage cheese Visible Contributes to gingival inflammation and caries. Removable |
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normal pH
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6.5 to 7.0
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what contributes to gingival inflammation
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food debris, biofilm, calculus
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