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14 Cards in this Set
- Front
- Back
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Uses |
To identify the microorganism. Antibiotic susceptibility testing. |
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Basic constituents |
Water Electrolyte, Peptone Meat extract Blood/serum Agar |
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Peptone |
- Mixture of partially digested proteins - Obtained from lean meant, proteins like heart muscle, casein or fibrin, digested with proteolytic enzymes. - Contains proteoses, amino acids, polypeptides, phosphates, minerals and accessory growth factors. - Special types - neopeptone and proteose peptone |
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Accessory growth factors |
Nicotinic acid, Riboflavin |
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Meat Extract |
Available as lab lemco Contains protein degradation products, inorganic salts, carbohydrates, growth factors. |
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Blood |
Used for enriching the media 5 to 10% defibrinated sheep blood is used. |
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Agar |
Prepared from sea weed/algae - geladium species. Contains long chain polysaccharide, protein like material and inorganic salts. Concentration - 2 to 3% Melts at 98°C Solidifies at 42°C Does not provide nutrition but acts as a solidifying agent. |
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Brain-Heart Infusion |
For the culture of non fastidious organisms. Recommended for culture of Streptococcus pneumoniae for bile solubility test. Contains brain and beef heart, peptones, glucose, nacl, buffers. Also used for preparing inocula for antibiotic sensitivity test |
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Classification based in physical state |
Liquid, Semisolid, Solid |
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Basis of presence of oxygen |
Aerobic, Anaerobic |
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Based on nutritional factors |
Simple, , Complex,Synthetic,Special Complex,Synthetic,Special Synthetic, Special |
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Types of special media |
Enriched, Enrichment, Selective, Differential, Indicator, Transport, Sugar |
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Simple media |
- Nutrient media is a basal media. - Contains peptone water, meat extract - 1% - addition if 0.5% of glucose makes it a glucose broth. - addition if 2-3% agar makes it nutrient agar. - used for simplest and routinely employed medium in laboratory diagnosis. - if concentration of agar is reduced to 0.2 to 0.4% it becomes semisolid and enables motile bacteria to spread. |
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Blood Media |
Haemolysis can be observed. |
