Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
13 Cards in this Set
- Front
- Back
|
LJ medium |
Lowenstein Jensen culture is a growth medium used for mycobacterium especially mycobacterium tuberculosis . Used for diagnosing mycobacterial infections. Also used for testing antibiotic susceptibility and differentiating different species of mycobacteriae |
|
|
Composition of LJ medium |
Malachite green - to prevent growth of other bacteria Glycerol- helps in growth of mycobacterium tuberculosis Asparagine - nitrogen coagulated eggs - fatty acids, protein mineral salt solution. Low levels of penicillin and nalidixic acid to prevent other bacterial growth. |
|
|
Culture media for myco. bovis |
LJ medium with sodium pyruvate instead of glycerol |
|
|
LJ Medium color |
Green,opaque and opalescent. |
|
|
Management of LJ medium |
Chk medium after 5-7 days of inoculation. Till 8 weeks. Mycobacterium colonies are colorless dry and rough.
|
|
|
Common ingredients of culture media |
Water, agar, peptone, malt , blood, serum |
|
|
Phases of growth media |
Liquid ( broth) solid ( agar), semisolid |
|
|
Phases of growth media |
Liquid ( broth) solid ( agar), semisolid |
|
|
Types of nutrition in culture media |
Simple, complex, synthetic |
|
|
Phases of growth media |
Liquid ( broth) solid ( agar), semisolid |
|
|
Types of nutrition in culture media |
Simple, complex, synthetic |
|
|
Special media |
Enriched, enrichment, selective, indicator or differential, transport, sugar |
|
|
Types of reducing media |
Liquid, solid, semisolid |
