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Test for proteins
Add biuret reagent
From blue to lilac if protein
Reducing sugar test
Add benenicts
Non reducing sugar test
Add dilute hcl and boil in water bath 2 min
Neutralise with sodium hydrogen carbonate
Do benedicts test
Starch test
Add iodine solution
Blue black means starch
Orange means no starch
Lipid test emulsion test
Add ethanol . Shake
Describe how concentration of glucose may be determined . The solution that goes into colorimeter
More reducing sugar present, more presipitate formed; more benedicts ( copper sulfate) used up; if precipitate filtered out; conc of remaining solution says how much benedicts used up
The lower the conc of filtered solution , the higher the conc reducing sugar present
Describe how calorimeter works
Shines beam of light
Photoelectric cells gives reading of how much light has passed through
Water calibrates calirometer 100% transmission and 0 absorbtion
Plot transmission against reducing sugar conc to get calibration curve
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