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Connective tissue is the most diverse type of tissue in the body and is composed of: fibrous material only, few cells and much extracellular material, acellular gelatinous substance, numerous cells with little intercellular tissue
few cells interspersed in large amounts of amorphous extracellular material called ground substance
areolar connective tissue is AKA: mineralized, dense, loose, specialized
loose or areolar connective tissue is widely distributed throughout the body, providing structural support and nourishment for cells and tissues.
connective tissue proper refers to tissue composed of: spongy cancellous and cortical, haline and fibrocartilage, hematopoietic bone marrow, collagen reticulin and elastin.
CNT proper is collagen, reticulin, and elastin. all other choices are specialized connective tissue.
solutions of acid aniline dyes in picric acid are used to demonstrate: reticulin, fibrocartilage, collagen, elastin
collagen: staining of collagen by these mixtures depends on the selectivity of collagen for acid dyes from strongly acid solutions.
in masson trichrome, after staining with Biebrich scarlet-acid fuchsin, sections are differentiated in: 1% HCL in 70% EtOH, dilute acetic acid, phosphomolybdic-phosphotunngstic acid, perchloric acid
phosphomolybdic-phosphotungstic acid.. it allows decolrization of collagen fibers which are subsequently stained with aniline blue.
with the verhoeff-van gieson technique, elastic fibers stain: red to purple, blue-black to black, yellow to brown, blue to blue-green
blue-black to blue with hematoxylin-ferric chloride-iodine solution.
in staining methods for reticulin, potassium permanganate, phosphomolybdic acid, and periodic acid function as: reducers, oxidizers, sensitizers, toners
oxidizers: potassium permanganate in Snook, Foot, and Gomori. phosphomolybdic acid in Wilder technique, and periodic acid is used in the Gridley technique.
the heme of choice for nuclear staining lengthy and acidic trichrome methods is: alum, phosphotungstic acid, chrome-alum, iron
iron-mordanted hematoxylins, such as Weigert's, are generally used for lenghty, acidic procedures because they are resistant to decolorization.
Although the Pas is not a conventional stain for reticulin, under certain conditions, these fibers are PAS positive and they are also: stainable with neutral red, stainable with orcein, argyrophilic, basophilic
argyrophilic: reticular fibers can be demonstrated with silver impregnation methods, using an artificial reducer.
in the verhoeff-van gieson technique for elastic fibers, collagen stains: red, blue, black, yellow
red: collagen stains red with acid fuchsin in the van gieson counterstain. the cytoplasm of muscle cells and other tissue elements stain yellow with the picric acid component of van gieson counterstain.
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