term1 Definition1term2 Definition2term3 Definition3
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nonspecific stain
-react with all microbes in an identical fashion
-useful solely for increasing contrast so that morphology, size, and arrangement of organisms can be determined
specific stain
give varying results depending on the organism being treatment
stains are chemicals containing
chromophores
groups that impart color
specificity is determined
by their chemical structures
basic dye
a stain that is cationic and will react with material that is negatively charged
surface of bacteria at a neutral pH is somewhat _________charged and will attract _________ dyes
negative charge
basic dyes
basic dye examples
crystal violet
safranin
basic fuchsin
methylene blue
acid dyes
-negatively charged chromophores and are repelled by the bacterial surface
-stain the background and leave the microbe transparent
acid dye examples
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