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Serial Dilution
Method of reducing the amount of bacteria in a particular medium by transferring bacteria from 1 tube into another tube
Dilutes the quantity of bacteria from the original tube
Why is serial dilution performed?
1. To isolate individual colonies
2. To quantify the bacteria in order to estimate the amount in the original colony
Countable plate
An agar plate that contains between 30 and 300 colonies of bacteria
Categories of non countable plates
1. No Growth (NG)
2. Too Few to Count (TFTC)
3. Too Numerous to Count (TNTC)
NG
No Growth
0 colonies per plate
TFTC
Too Few to Count
Less than 30 colonies per plate
TNTC
Too Numerous to Count
More than 300 colonies per plate
What machine can be used to accurately view the exact number of colonies on a plate?
Quebec Colony Counter
How should one count bacterial colonies on a Quebec colony counter?
- snake back and forth in rows
- count only the bacteria in the box, on the top line, and on the front/preceding line
CFU
Colony Forming Units
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