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What is it?
-Method to separate DNA fragments by using electricity
-DNA had - charge to it moves towards + pole
-small pieces move faster that large pieces
-loading dye= mix of acidic dyes (- ion is colored) and sucrose
+does not bind to DNA, just moves with it
+ sucrose will make sample heave so it will sink
+dyes = xylene cyanol ( blue green) moves like big DNA, bromophenol blue (purple) moves like small DNA
-DNA ladder- mixture of DNA fragments with known size- ethidium bromide or gel red stains that stick to DNA and glow in UV light hind 3
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