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What is the purpose of gas chromatography?
To separate and analyse components of a mixture on the basis of boiling point (volatility).
What happens to molecules in a mixture of substances when a sample is injected into a GC instrument?
Converts into gas, molecules vaporised and carried by inert gas into column.
What happens to the molecules in a mixture of substances after the injection of the sample?
Molecules travel through column and mixture separates into individual components on the basis of different affinity of molecules with stationary phase.
What happens to sample molecules as they are introduced into a mass spectrometer?
Molecules are blasted with electrons which cause them to break down into positively charged fragments.
What do the signals in a mass spectrum represent?
Mass of fragmented ions.
How can unknown compounds be identified using MS?
Similarity search databaseFragmentation pattern (?) is used to solve chemical puzzle of unknown compound.
Explain what GCMS is being used to determine in this demonstration?
Testing purity of aspirin, monitor reaction progress and outcome of reactions.
Describe other applications of GCMS
-Toxicity analysis of food or environmental samples-Crime scene analysis in forensic science.
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