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Effect of temperature
Increases the kinetic energy, they kove faster and form more e-s complexes.
Temperature denatured hydrogen and ionic bonds, changing the shape of the active site.
Effect of pH
Changes the charges in the active site, preventing induced fit from happening.
Breaks hydrogen bonds changing the tertiary structure.
Effect of enzyme concentration
Increases the rate of reaction up to a point then the substrate concentration becomes a limiting factor.
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