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16 Cards in this Set

  • Front
  • Back

Which way is the code read in proteins?

5'-3' or N-C

What method was used to decipher the triplet code?

In vitro translation assay

What are the 3 stop codons?

UAA


UAG


UGA

How can you tell the genetic code is a non overlapping triplet code?

3 mutations are needed to restore the reading frame

What advantage is there to a degenerate code?

Creates a tolerance to mutations

What is the adapter in translation?

tRNA

Where does the amino acid attach in a tRNA molecule?

3' and 5' end

What is wobble base pairing?

The way tRNA can recognise more than one codon because of variations in allowed patterns of hydrogen bonding

What is the purpose of the 2 ribosome subunits

Peptide bind formation


Codon matching

Name the 3 binding sites on a ribosome

A - aminoacyl tRNA


P - peptidyl- tRNA


E - exit

Name the 4 steps in protein translation

1. Complementary tRNA binds to mRNA codon


2. Peptide bond forms between previously bound amino acid and the current one


3. Large subunit translocation


4. Small subunit translocation

What catalyses the formation of peptide bonds

Peptidyl transferase

What type of reaction occurs in the formation of peptide bonds?

Condensation

How is translation ended? (3)

1. Stop codon moves into A site


2. Release factor binds into A site


3. Water molecule added to the end of the amino acid chain

How long does it take to transcribe a 10kbp gene?

10 mins

How long does it take to translate a 300 amino acid protein?

1 min