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Immunohematology:

Criteria for Donor Selection
Age- 17 yrs. min., no max
Temp- ≤ 37.5˚C or ˚≤ 99.5˚F
BP- ≤ 180 syst., ≤ 100 diast.
Hb- ≥12.5g%
Hct- ≥38%
Pulse- 50-100 beats/min.
Weight- min. 110 lb / 50 kg
Immunohematology:

Donor Defferal - General
1 year
Possible exposure to hepatitis, HIV or malaria
3 year
Possible exposure to malaria (visitor/migrant/prev.diagnosis)
Indefinite
Defiinite disease or habits strongly associated with bloodborne pathogens
Immunohematology:

Donor Deferral - 1 year
Hepatitis B Immune Globulin
Poss. exp. to hepatitis, HIV or malaria
Tattoo
Mucous membrane exposure to blood
Skin penetration with instruments contaminated by blood/body fluids
Sexual contact with symptomatic individual for any viral hepatitis, confirmed + for HBsAg / HIV or in high risk category
After completed therapy for syphilis, gonorrhea or reactive STS
Traveled to endemic area for malaria w/wo antimalarial drugs, free of symptoms
> 72 hrs. in correctional institution
*malarial parasites only in red cells, plasma preps exempt
Immunohematology:

Donor Deferral - 3 years
Asymptomatic-
Visitor / migrant from area endemic for malaria
Previously diagnosed with malaria
*malarial parasites only in red cells, plasma preps exempt
Immunohematology:

Donor Deferral - Indefinite
Viral Diseases:
Viral Hepatitis after age 11
+ confirmatory test for HBsAg
Repeatedly reactive test for anti-HBc
Donated only unit to recipient who developed post transfusion hepatitis, HIV or HTLV
Present/Past infection of HCV, HTLV or HIV
Evidence of parenteral drug use
Other Diseases:
Received dura mater or pituitary growth hormone of human origin, family history of CJD or risk of vCJC
History of Chagas' disease, babeiosis
Immunohematology:

Maximum Collection of Whole Blood
no more than 10.5 mL of whole blood per kg. of body weight, including samples
Immunohematology:

Wating Period for Whole Blood Donation
56 days
Immunohematology:

Common Blood Anticoagulants, Additives & Solutions
CPD - anticoagulant
citrate, phosphate, dextrose
21 day expiration
CPDA-1 - anticoagulant
cpd & adenine
35 day expiration
Additives(enhance rbc survival)
contain dextrose, adenine, sodium chloride among others
42 day expiration
Rejuvenating Solutions
contain pyruvate, inosine, phosphate and adenine
Restores 2, 3-DPG and ATP
added during storage or up to 3 days after expiry
can freeze unit or store at 1-6˚C if using within 24 hrs.
(must wash cells before use)
Immunohematology:

Autologous Donations
no age limit
Hct - 33%, Hb 11g/dL
No bacteremia
Must be labeled "for autologous use only"
Segregated from allogenic units
Immunohematology:

(Autologous Only)
Low Volume Donations
Volume to Draw formula
ex. 90 lb donor

(90 lb/110 lb) x 450 ml (standard) = 368 ml
or
(donor wt. in kg/50) x 450
Immunohematology:

Low Volume Collections
If 300-404 mL drawn, label as
Red Blood Cells Low Volume

If < 300 mL drawn, adjust coagulant

*components may not be made from these units
Immunohematology:

Amount of Anticoagulant for
Low Volume Collections
368 x 14% = amt. of anticoagulant
368 x .14 = 51.5 (52 ml) needed
63 (standard) - 52 = 11 ml to remove from primary bag into attached satellite bag prior to draw
Immunohematology:

Hemapheresis/Apherisis
Criteria
Donor criteria same as Whole
Waiting Period of 48 after apheresis to donate Whole
max 500 mL (600 mL if > 175 lbs.
Limits # of donor exposures
Immunohematology:

Hemapheresis/Apherisis
Methods
Centrifugation
Withdrawal of whole blood, remove selected fraction and remaining reinfused into donor
Filtration
removal of only plasma through a membrane
Adsorption
removal of only a selected constituent of plasma with reinfusion of remaining
Immunohematology:

Apheresis Types
Cytapheresis - cells
Plasmapheresis - plasma
Plateletpheresis - platelets
Leukapheresis/ Granulocytapheresis - leukocytes/granulocytes
Immunohematology:

Donor Criteria for Apheresis
Same as for Whole Blood

Age - 17 yrs. min.
Temp. - ≤ 37.5 C or ≤99.5 F
BP - ≤ 180 sys/ ≤ 100 dias
Hct - ≥ 38%, Hb ≥ 12.5g%
Pulse - 50 - 100 beats/ minute
Wt. - min. 110 lb / 50 kg
Immunohematology:

Temperature Conversion
US to metric
Metric to US
Tc = (5/9) x (Tf - 32)
Tf = (9/5) x Tc + 32
Immunohematology:

Tests Performed on Donor Blood
ABO
Rh
Antibody Screens
Syphilis
Viral Diseases
Immunohematology:

Blood Components
Whole Blood
RBCs
Washed RBCs
Leukocyte Reduced RBCs\
Frozen Cells
Fresh Frozen Plasma
Cryoprecipitate
(Cryoprecipitated Antihemophilic Factor)
Factor Concentrates
- Factor VIII
- DDAVP
- Factor IX
- Factor VIII
- Factor VIII with vWF
Platelets
Granulocytes
Irradiated Blood and Components
Immunohematology:

Whole Blood
Use, Standards, and Storage
Very Rarely Given
Severe Shock
(Blood Loss ≥ 25% blood volume)
Need RBCs for oxygen and Plasma for volume
Hct - 80%
1-6˚C (Closed System)
21 days (CPD)
35 days (CPDA-1)
42 days (Additive)
1-6˚C (Open System)
24 hours