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60 Cards in this Set
- Front
- Back
Name the test and what it is for
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Catalase test
Differentiate: - Staphylococcus (+) from Streptococcus (-) - Among small non-branching, non-spore forming aerobic gram positive bacilli: ( + ) Listeria spp. Corynebacterium spp. ( - ) Erysipelothrix rhusiopathiae (H2S+), Arcanobacterium haemolyticum |
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Name the test and what it is for
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Coagulase tube test: detects free coagulase producing organisms
Coagulase-positive staphylococci: Staphylococcus aureus subsp. anaerobius S. a. aureus S. a. delphini S. hyicus S. intermedius (dog bite) S. lutrae Staphylococcus schleiferi subsp. coagulans Coagulase-negative staphylococci: S. saprophyticus S.cohnii subsp. cohnii S. cohnii subsp. urealyticum S. captitus subsp. captitus S. warneri S.hominis S.epidermidis S. caprae S. lugdunensis (+ with bound/slide coagulase test and PYR+) Coagulase test = Rabbit plasma + organism incubated at 35*C and checked at 4 hr and 24 hr |
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Name the test and what it is for
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Coagulase slide test: detects bound (clumping factor) coagulase producing organisms
S. aureus group and S. lugdunensis ( - coagulase tube test) |
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Name the test and what it is for
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Coagulase latex agglutination test: detects bound (clumping factor) coagulase producing organisms
S. aureus group and S. lugdunensis ( - coagulase tube test) |
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Name the test
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Kirby-Bauer disk diffusion antibiotic sensitivity testing showing nosocomial MRSA
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Name the test
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Kirby-Bauer disk diffusion antibiotic sensitivity testing showing community acquired MRSA with positive D-test
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Novobiocin disk/gram stain, name the organism on the right side
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Staphylococcus saprophyticus
Coagulase - Staph. involved in approximately 10-20% of UTI (young females) |
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Name the probable organism
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Clostridium perfringens showing double zone of Beta hemolysis on SBA
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Lecithin Lactose Agar
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Clostridium perfringens precipitates lecithin around colonies (opaque halo)
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thioglycolate broth
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Clostridium perfringens in thioglycolate broth, showing gas production
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Name organism
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Pseudomonas aeruginosa on BAP showing metallic haze colonies
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Mac Conkey agar
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Pseudomonas aeruginosa on MAC
Glucose non-fermenter, oxidase positive |
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KIA/TSI tube, organism?
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K/K:
non-glucose, non-lactose, non-sucrose fermenter No H2S Pseudomonas |
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Type of hemolysis, possible organisms
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Beta-hemolysis
Groups A (pyogenes) C (S. dysgalactiae Subs. equisimilis) G (S. equi Subs. zooepidermicus) B (pyogenes) β-hemolytic enterococci |
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Bacitracin test
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Group A Streptococcus (pyogenes) is susceptible to Bacitracin
Bacitracin resistant Beta-Hemolytics S. milleri group (F) S. dysgalactiae Subs. equisimilis (C) S. equi Subs. zooepidermicus (G) β-hemolytic enterococci |
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PYR test
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Positive in Streptococcus pyogenes (GAS)
AND Beta-hemolytic Enterococci |
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Lancefield testing caveat
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Does not distinguish between large-colonyforming,
pyogenic streptococci of groups A, C and G and small-colony-forming group A, C and G strains of the anginosus or “S. milleri” group |
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This bacterium grew in Regan-Lowe medium forming round, domed, mercury-silver, colored, shiny colonies
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Bordetella pertussis
Culture sensitivity: • If ≥14 days from onset of cough, sensitivity is lower, so serology is sent instead of culture • Caveat: Serology is uninterpretable in immunized or unimmunized patients <11 years of age – These patients are cultured regardless of duration of symptoms |
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Name organism
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Corynebacterium diphtheriae
Non-motile Catalase + |
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Name organism and medium
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Tellurite-containing medium, black colonies of Corynebacterium diphtheriae
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Optochin Susceptibility Test, name organism
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Streptococcus pneumoniae
Optichin sensitive, > 14 mm. inhibition |
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BHI agar, what is preferable for this organisms?
Name organisms: Green arrow Red arrow Blue arrow |
Preferable medium for Haemophilus spp.:
Chocolate agar or Horse blood agar with free X (hemin) and V (NAD) Red (X and V) H. influenzae H. haemolyticus Green (V only) - ("The P's like the V") H. parainfluenzae H. paraheamolyticus Blue (X only) - ("X-Rated") H. ducreyi |
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S. aureus streak
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Haemophilus spp. satellitism
S. aureus produces V and if Beta-hemolytic releases X from SBA |
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Gram + methylene blue: name organism
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Corynebacterium diphteriae
Metachromatic granules with methylene blue |
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BYCE medium, name organism
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Legionella pneumophila
Buffered Charcoal Yeast Extract agar Legionella won't stain with gram on direct specimens, it will from subculture specimens |
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Facultative anaerobes, Acid Fast negative, organism group?
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Actinomyces spp.
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Molar tooth colonies
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Actinomyces israelii
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Acid fast stain
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Nocardia spp
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Chalky-white colonies when young then yellow/orange colonies
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Nocardia asteroides
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Medusa-head colonies
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Bacillus anthracis
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Name organism
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Bacillus anthracis
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Name organism
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Bacillus anthracis
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Optichin disks
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Resistant
PYR positive, Bile esculin positive: Enterococcus spp. PYR negative: Bile esculin positive: S. bovis (D) Bile esculin negative: Viridans |
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Ampicillin Resistant
Vanco Sensitive |
Enterococcus faecium non-VRE
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Ampicillin Vancomycin Resistant
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Enterococcus faecium VRE
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Ampicillin, Vancomycin susceptible
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Enterococcus faecalis
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Quad plate
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Test for synergy of aminoglycosides with vancomycin for Enterococci causing bacterial endocarditis
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Urease test, gram and tissue, name organism
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Brucella melitensis
Castaneda biphasic blood culture held for 21 days historically used for culture |
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Common-antigen test (glutamate
dehydrogenase or GDH test) |
Used as a screening test for C. diff. to decide which
specimens to evaluate using another assay (e.g., cytotoxicity assay or toxin EIA) |
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Glucose fermenting
Nitrate to nitrite Oxidase negative Possible organisms with this colony type in Mac Conkey |
Lactose fermenting
E. coli (indole+) Enterobacter spp. (VP test +, catalase+, citrate+) Klebsiella spp. (VP test +, non-motile, mucoid colonies) |
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Hektoen enteric agar
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E. coli
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Hektoen enteric agar
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Salmonella spp.
H2S production |
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KIA-TSI
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E. coli
Enterobacter Klebsiella Acid Slant/Acid Butt (A/A) Shigella Vibrio Alkaline Slant/Acid Butt (K/A) Salmonella Citrobacter Proteus Alkaline Slant/Acid Butt (K/A) + H2S Pseudomonas Alkaline Slant/Alkaline Butt (K/K) |
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CIN agar
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Yersinia enterocolitica
•Septicemia in iron overload syndromes •Mesenteric adenitis – RLQ pain mimics appendicitis •Grows well at 4*C (like Listeria) •CIN agar (Cefsulodin-irgasan-novobiocin) |
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Safety pin morphology
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Yersinia pestis
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TBCS agar
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Vibrio cholerae
Oxidase positive/glucose fermenter KIA: K/A TCBS (Sucrose) Yellow: V. cholerae Green: V. vulnificus, V. parahaemolyticus |
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Gram, identify organism group
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Vibrio spp
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Special BAP medium, at 42°C and @ 5% O2, 10% CO2, 85% nitrogen
• Catalase positive • Oxidase positive |
Campylobacter spp
• Hippurate hydrolysis test – If positive, identifies isolate as C. jejuni – Of Campy species, only jejuni is positive |
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Name organism
Motile |
E. coli
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MUG test positive (β-glucuronidase activity)
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E. coli
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Name organism
Non-motile VP+ |
Klebsiella spp
Indole: + K. pneumoniae - K. oxytoca |
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Name organism
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Proteus spp
Indole: + P. vulgaris - P. mirabilis |
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Name organism
Coagulase - Novobiocin resistant |
Staphylococcus saprophyticus
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Name organism
Thayer Martin Glucose fermenting |
Neisseria gonorrheae
Molecular amplification methods – Permit concurrent detection of CT in single specimen – Allow use of urine as a specimen (non-invasive) – Do not require viable organisms (transport not an issue) – Highly sensitive – Rapid • Disadvantages: – GC DNA may be present up to 3 weeks after successful treatment, so NAATs should not be used as test of cure – Isolates not available for susceptibility testing if treatment difficulties arise – Results inadmissible evidence in medicolegal cases |
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Blue
Pink Yellow Green Magenta |
Blue: GBS
Yellow: Listeria monocytogenes Pink: Pneumococcus Green: Neisseria meningitidis Magenta: H. influenzae |
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Name organism
Thayer-Martin Ferments maltose |
Neisseria meningitidis
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Lazy-Beta hemolysis
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Streptococcus agalactiae
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Name organism
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Streptococcus agalactiae
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Name organism
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Listeria spp.
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Name organism
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Listeria monocytogenes
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