Clinical Chemistry Cls 2211 Unit 6 Electrolytes

Front 1

Explain the principle of Atomic absorption spectrophotometry

Back 1

- vaporized elemental metals in their "ground state" can absorb photons of light given off by identical atoms returning to their "ground state" from their "excited state"
- Excitation is a problem- to avoid use a cooler flame, dilute all specimens controls, and calibrators

Front 2

Explain the principles of flame photometry

Back 2

Vaporized 1A elements in their ground state can gain energy and move into their excited state
- they return to their ground state by releasing energy as a photon of light with a wavelength specific to that element

Front 3

Flame photometry sources of error

Back 3

Self absorption
- dilution limits this error

Front 4

Principle of ISE

Back 4

-Ion selective electrodes
- activity of electrolyte on one side of an electrode membrane, as compared to a fixed concentration of that electrolyte on the other side causes a electrical potential to be built up across the membrane.

Front 5

Reference electrode

Back 5

Calomel- electrode filled with Hg and HgCl2 paste

Front 6

pH ISE glass electode

Back 6

pH sensitivity due to a special glass that is sensitive to H+ ions
- HCl is the filler inside the electrode

Front 7

Na+ ISE glass electode

Back 7

Na+ sensitivity due to glass that is sensitive to Na+
- NaCl is filler in electrode

Front 8

K+ ISE glass electode

Back 8

K+ specificity due to valinomycin coating glass that binds to K+ ions
- KCl is filler in electrode

Front 9

NH4+ ISE glass electode

Back 9

NH4+ specifiicity due to Monactin and Nonactin coating glass that binds NH4+ ions
- NH4Cl is filler in electrode

Front 10

Li+ ISE glass electode

Back 10

Li+ specificity due to proprietary ionophore that is embeddded within the glass membrane giving specificity by binding Li+ ions
- LiCl is filler in electrode

Front 11

How to tell if your getting an ISE error

Back 11

you get Slow, irratic, irreproducible, unstable response

Front 12

Total CO2 measurer

Back 12

Severinghaus- for total CO2

Front 13

■ admonish

Back 13

v.
warn reprove.
"When her courtiers questioned her religious beliefs, Mary Stuart admonished them, declaring that she would worship as she pleased."