Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
8 Cards in this Set
- Front
- Back
Lysis
|
Breaking down of a cell, often by viral, enzymic, or osmotic mechanisms that compromise its integrity.
Done by centrifugation. Yield a crude extract. |
|
Column chromatograph
|
Apply protein mixture to resin or matrix, interacts differently with various protein
|
|
Purpose of adding buffer
|
Washing away any proteins that do not bind to the matrix
Another is added to dissociate the matrix |
|
Elution buffer are chosen
|
so that different proteins dissociate from the matrix
|
|
Ion exchange chromatograph
|
resin in cation or anion groups
|
|
Proteins are eluted (Ion exchange chromatograph)
|
depends on the nature of charged amino acid residues
|
|
Gel-exclusion chromatograph
|
Proteins are separated by size
Resin is composed of hollow beads with pores Large proteins move around the beads and elute earlier Small proteins can enter the resin pores and take a longer path through the column |
|
Affinity chromatograph
|
Proteins are sorted by the type of ligand, covalently coupled to the column resin
|