Chromatography

Front 1

What is CHROMATOGRAPHY

Back 1

A technique used to analyse and separate a mixture of coumpounds into individual components.

Front 2

What are the phases in Chromatography?

Back 2

STATIONARY phase

MOBILE phase

Front 3

How is separation achieved?

Back 3

This is achieved by the distribution of the molecules in the stationary and mobile phase.

Front 4

TURE/ FALSE

Chromatography can be linked with other methods of analysis eg HYPHENATED Technique.

Back 4

True

Front 5

Names of the types of Chromatography.

Back 5

ADSORPTION Chromatography. E.g Thin Layer Chromatography (TLC) / Lab-Scale Column

PARTITION Chromatography. E.g High Performance Liquid Chromatography.

ION EXCHANGE Chromatography.

GEL PERMEATION Chromatography.

AFFINITY Chromatography.

Hint 5

1. Stationary phase is solid e.g silica, alumina.

2. Both stationary and mobile phases are liquids.

3. Stationary phase is a ion exchange resin

4. Size exclusion chromatography

5. Ligand immobilised on solid stationary phase

Front 6

TURE/ FALSE

TLC has a Silica stationary phase.

Back 6

True

Front 7

TRUE/ FALSE

The Silica stationary phase has non-polar compound eluted first.

Back 7

True

Front 8

TRUE/ FALSE

Results from a TLC can be visualised using UV lights, iodine, sulphuric acid, molybdate.

This results are qualitative

Back 8

TRUE

Hint 8

Front 9

What is a Lab-Scale Chromatography?

Back 9

Separation of organic compounds on an INERT stationary phase. E.g. silica or alumina.

This gives qualitative results

Hint 9

TLC must be carried out before using COLUMN throughout to detect eluted compounds

Front 10

TLC and COLUMN is an example of which type of chromatography?

What type of results does it give?

Back 10

TLC AND COLUMN are Adsorption Chromatography.

This gives qualitative results

Front 11

What type of chromatography gives is a Quantitative Technique which gives Quantitative Results?

Back 11

PARTITION Chromatography.

E.g. HPLC- mobile phase is Liquid.

Gas Chromatography- mobile phase is Gas

Hint 11

Analytes are separated based on the differing AFFINITIES for the stationary and mobile phase.

Front 12

Chromatogram

Back 12

Need to copy down

Front 13

What is High Performance Liquid Chromatography?

Back 13

Chromatography involves mixture being separated on the column and each of the components are detected using means.

Sample is inserted into a solution on to the column.

Front 14

TRUE/ FALSE

The stationary phase consisting of silica particles is packed into stainless steel Columns under high pressure

Back 14

TRUE

Hint 14

Stationary phase can be modified to allow separation of different molecules.

E.g. chiral stationary phase used to separate enantiomers.

Front 15

Which HPLC silica for the stationary phase is more polar?

NORMAL phase / REVERSE phase HPLC

Back 15

Normal HPLC

Front 16

How is stationary phase (silica) modified to become less polar?

Back 16

With the addition of C18 alkyl groups of the silica surface.

(Octadecylsilyl,ODS).

Front 17

TRUE/ FALSE

In reverse phase HPLC the columns are differentiated.

Columns depends on the analytes to be separated.

Back 17

TRUE

Hint 17

E.g. analytes that are NON- polar or less polar will be retained longer.

Front 18

Fg

Back 18

Gy