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26 Cards in this Set

  • Front
  • Back

Bone and tendons for transplant are decontaminated by

Supercritical fluids.

Glutaraldehyde is considered one of the most effective disinfectants for hospital use. Which of the following statements about glutaraldehyde is false?

. Stains and corrodes

If you were preparing nutrient agar at home and didn't have an autoclave, what could you use to sterilize the nutrient agar

Pressure cooker at 121°C for 15 minutes

Iodophors differ from iodine (I2) in that iodophors

All of the above.

Place the following surfactants in order from the most effective to the least effective antimicrobial activity:
1-Soap; 2-Acid-anionic detergent; 3-Quats.

3-2-1

A disk-diffusion test using Staphylococcus gave the following results:

Disinfectant Zone of inhibition (mm)
A 0
B 2.5
C 10
D 5


In Table 7.1, which compound was the most effective against Staphylococcus?

10

Table 7.2
The fate of E. coli O157:H7 in apple cider held at 8°C for 2 weeks, with and without preservatives, is shown below:

Bacteria/ml
Cider only 2.2
Cider with potassium sorbate 2.0
Sodium benzoate 0
Sorbate + benzoate 0


In Table 7.2, which preservative is most effective?

benzoate

Table 7.3


The following data were obtained by incubating gram-positive bacteria in nutrient medium + disinfectant for
24 hr, then transferring one loopful to nutrient medium (subculturing). (+ = growth; - = no growth)

Doom K.O.
Dilution Initial Subculture Initial Subculture
1:16 - + + +
1:32 - + + +
1:64 - + + +
1:128 + + + +

Mortum Sterl
Dilution Initial Subculture Initial Subculture
1:16 - - - +
1:32 - + + +
1:64 + + + +
1:128 + + + +


In Table 7.3, which disinfectant was bactericidal?

mortum

Table 7.3


The following data were obtained by incubating gram-positive bacteria in nutrient medium + disinfectant for
24 hr, then transferring one loopful to nutrient medium (subculturing). (+ = growth; - = no growth)

Doom K.O.
Dilution Initial Subculture Initial Subculture
1:16 - + + +
1:32 - + + +
1:64 - + + +
1:128 + + + +

Mortum Sterl
Dilution Initial Subculture Initial Subculture
1:16 - - - +
1:32 - + + +
1:64 + + + +
1:128 + + + +


In Table 7.3, which disinfectant is the most effective at stopping bacterial growth?

doom

The antimicrobial activity of chlorine is due to which of the following?

The formation of hypochlorous acid

Which concentration of ethanol is the most effective bactericide?

70%

Which of the following could be used to sterilize plastic Petri plates in a plastic wrapper?

Gamma radiation

Which of the following does NOT achieve sterilization?


Formaldehyde


b. Ethylene oxide


c. Autoclave


d. Pasteurization


Correct


e. Dry heat

Pasteurization

Which of the following does NOT directly result from application of heat?


Select one:


a. Breaking of hydrogen bonds


b. Denaturing of enzymes


c. Damage to nucleic acids


d. Cell lysis


e. Breaking of sulfhydryl bonds

Cell lysis

Which of the following is a limitation of the autoclave?


Select one:


a. Cannot be used with glassware


b. Requires a long time to achieve sterilization


c. Cannot kill endospores


d. Cannot be used with heat-labile materials


e. Cannot inactivate viruses

Cannot be used with heat-labile material

Which of the following is NOT a factor contributing to hospital-acquired infections?


Select one:


a. Some bacteria metabolize disinfectants.


b. Invasive procedures can provide a portal of entry for bacteria.


c. Gram-negative bacteria are often resistant to disinfectants.


d. Bacteria may be present in commercial products such as mouthwash.


e. All of the above may contribute to hospital-acquired infection.

All of the above may contribute to hospital-acquired infection.

Which of the following is NOT used to disinfect water?


Select one:


a. Ozone


b. Heat


c. Copper sulfate


d. Chlorine


e. Gamma radiation

heat

Which of the following substances is NOT effective against nonenveloped viruses?


Select one:


a. Alcohol


b. Ozone


c. Ethylene oxide


d. Chlorine


e. All are equally effective.

Alcohol

Which of the following substances is used for surgical hand scrubs?


Select one:


a. Chlorhexidine


b. Phenol


c. Chlorine bleach


d. Glutaraldehyde


e. Soap

Chlorhexidine

Which of the following treatments is the most effective for controlling microbial growth?


Select one:


a. 72°C for 15 sec.


b. 140°C for 4 sec.


c. 63°C for 30 min.


d. They are equivalent treatments.

They are equivalent treatments.

Which of the following will NOT destroy prions?


Select one:


a. Boiling


b. Proteases


c. Incineration


d. NaOH + autoclaving at 134°C


e. All of the above destroy prions.

Boiling

Which of the following will NOT preserve foods?


Select one:


a. Ionizing radiation


b. Desiccation


c. Osmotic pressure


d. Microwaves

microwaves

Which of these disinfectants acts by denaturing proteins?


Select one:


a. Aldehydes


b. Alcohols


c. Phenolics


d. Bisphenols

Bisphenols

Which one of the following does NOT belong with the others?


Select one:


a. Glutaraldehyde


b. Propylene oxide


c. Hydrogen peroxide

Hydrogen peroxide

Which one of the following is most resistant to chemical biocides?


Select one:


a. Gram-negative bacteria


b. Protozoan cysts


c. Mycobacteria

Mycobacteria

Which one of the following pairs is mismatched?


Select one:


a. Ozone takes electrons from substances


b. Ionizing radiation hydroxyl radicals


c. Plasma sterilization free radicals


d. Ultraviolet radiation desiccation

Ultraviolet radiation desiccation