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20 Cards in this Set
- Front
- Back
Southern blot
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protocol for transferring DNA sequences separated by gel electrophoresis onto a nitrocellulose filter paper for analysis by hybridization with a DNA probe
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allele-specific oligonucleotide (ASOs)
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short oligonucleotides that hybridize with alleles distinguished by a single base difference
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DNA fingerprint
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the multilocus pattern produced by the detection of genotype at a group of unlinked, highly polymorphic loci
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What does PCR stand for?
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polymerase chain reaction
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PCR is?
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a scientific technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence
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What does RFLP stand for?
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restriction fragment length polymorphism
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What does ASO stand for?
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allele-specific oligonucleotide
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fidelity
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accuracy; exactness
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amenable
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ready or willing to answer, act, agree, or yield; open to influence, persuasion, or advice; agreeable; submissive
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DNA fingerprint
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a pattern produced by the simultaneous detection of genotype at a group of unlinked, highly polymorphic loci.
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What cell was Dolly the sheep grown from?
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An adult udder cell
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Bolshevik Revolution
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a political revolution and a part of the Russian Revolution of 1917, which created the Soviet Union
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What is this called?:
a DNA sequence that occurs in two or more variant forms |
DNA polymorphism
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What is this called?:
combination of alleles at multiple tightly linked loci that are transmitted together over many generations |
haplotype
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What is this called?:
variation in the length of a restriction fragment detected by a particular probe due to nucleotide changes at a restriction site |
RFLP
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What is this called?:
a short oligonucleotide probe that will hybridize to only one allele at a chosen SNP locus |
ASO
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What is this called?:
two different nucleotides appear at the same position in genomic DNA from different individuals |
SNP
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What is this called?:
detection of genotype at a number of unlinked highly polymorphic loci using one probe |
DNA fingerprinting
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What is this called?:
DNA element composed of tandemly repeated identical sequences |
minisatellite
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What is this called?:
location on a chromosome |
locus
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