Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
8 Cards in this Set
- Front
- Back
|
What’s the difference between biotechnology & genetic engineering? |
Biotechnology uses microorganisms to make practical products while genetic engineering directly manipulates an organisms genome by using technology |
|
|
3 cycles of PCR |
1. Denaturation 2. Priming 3. Extension |
|
|
Define the denaturation step in PCR |
Exposure to heat separates the 2 strands of the target DNA |
|
|
Define the priming step in PCR |
A mix containing extra DNA primers that provide DNA polymerase w/ the 3’ hudroxyl group required for DNA synthesis |
|
|
Define the Extension step in PCR |
Increases the rate at which DNA polymerase replicates each strand to produce more DNA by increasing temp |
|
|
Describe some applications of biotechnology |
Scientists industrialized natural metabolic reactions of bacteria to make large quantities of acetone, butanol, & antibiotics. |
|
|
Describe some applications of genetic engineering |
1. To eliminate undesirable traits 2. Select for 2 culture cells w/ beneficial traits 3. Combine traits of 2 or more organisms 4. Create organisms that synthesize products |
|
|
What is DNA polymerase derived from hyperthermophillic bacteria use for PCR? |
A thermocycler automatically performa PCR by continuously cycling DNA polymerase derived from hyperthermophillic bacteria through 3 temperature regimes |
