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32 Cards in this Set
- Front
- Back
Light microscope
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Uses wave length of light to examine thin section of tissue
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Electron light microscope
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Look at detailed structures inside cell
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Fixation
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(e.g. formalin)
Fixes tissue in state it was in when it was removed Stops enzymes |
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Eosinophilic
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- stains pink
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Basophilic
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- stains blue/purple
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Connective tissue
Classification |
Embryonic connective tissue
Connective tissue proper Cartilage Bone Blood and lymph Haemopoeitic tissue |
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Connective tissue
Composition |
Composed of cells and extracellular (intercellular) material
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Extracellular material
Amorphous |
(shapeless – ground substance)
Fluid (blood, lymph) Jelly-like (vitreous humour) Solid (cartilage, bone) |
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Collagen fibers
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Strong, flexible
White fibrous tissue Eosinophilic in H&E Mostly type I collagen, but variation in specialized tissues |
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Reticular fibers
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Fine network
Poorly stained in H &E Black in reticulin stain Composed of varying combinations of collagen and extracellular glycoprotiens |
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Connective tissue proper
Functions |
Support
Protection Connection within locomotors system Holds tissue together Energy storage |
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Type of connective tissue
loose |
(higher ratio of cells to fibers)
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Type of connective tissue
Adipose |
(many fat cells)
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Type of connective tissue
Dense |
(lower ratio of cells to fibers)
Irregularly arranged Regularly arranged |
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Mesenchymal cells
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In embryonic tissues
Stellate (star shaped)- many cytoplasmic processes Oval/round nucleus |
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Fibroblasts
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Stellate or spindle shaped
Ovoid nucleus Abundant basophilic cytoplasm Synthesizes and secretes collagen and other extracellular matrix components Differentiates into fibrocyte Very active in protein synthesis and secretion |
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Macrophage
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Large cell
Derived from blood monocyte Phagocytic cell Produces lysosomal proteases (protein degrading enzymes), cytokines, prostaglandins (substances that mediate inflammatory reactions) |
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Fat cells (adipocytes)
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Accumulate fat droplets in cytoplasm
Fat droplets coalesce to form single large fat droplet, displacing nucleus to side of cell Energy storage, insulation and protection |
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Mast cell
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Scattered n low numbers in most connective tissues (often found close to blood vessels)
Cytoplasm filled with granules containing heparin (anticoagulant), histamine (increased blood vessel permeability), serotonin (vasoconstrictor) and proteases Degranulates when tissue damaged |
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Plasma cell
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B-lymphocyte actively producing antibody
Eccentrically placed round nucleus with chromatin radially arranged Abundant basophilic cytoplasm Negative image (Golgi apparatus) prominent |
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Melanocyte
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Pigment cell
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adipocyte
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mesencymal cell
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fibroblast
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fibrocyte
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macrophage
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mast cell
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plasma cell
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