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25 Cards in this Set
- Front
- Back
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Vesicle docking order
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1. tethering
2. docking 3. fusion |
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SNARE
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Soluble N-ethylmaleimide-sensitive factor Attachment REceptor
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Rab protein
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G protein (GTPase)
GTP -> GDP + P + Energy -similar to ran in nuclear protein transport -Peripheral -- is anchored to vesicle membrane via lipid anchor Rab-GTP complex can bind to tethering protein Rab-GDP complex released from tethering protein |
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Vesicle docking image
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rab has gtp
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Tethering protein
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v-SNARE
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attached to vesicle, winds with t-SNARE of target membrane
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t-SNARE
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part of vesicle's target membrane, winds with v-SNARE to bring vesicle into membrane
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tethering protein
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part of target membrane, attaches to Rab-GTP to bring vesicle to membrane
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what holds SNARE proteins together?
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Electrostatic charges of AA side chains; they wrap around one another
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SNARE proteins
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Docking
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when t and v SNAREs interact
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Exocytosis
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Movement of proteins, lipids, and carbohydrates out of cell via vesicles
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Membrane and secretory proteins must...
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...be modified and approved before reaching their final destination.
Cytoplasm proteins do not need to be modified |
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Glycosylation
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in ER and Golgi
-->transmembrane glycoprotein -important for stability (prevents degradation) -important part of cell's plasma membrane carbohydrate layer -protein can fold differently |
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Glycosylation -- cell surface (non-cytosolic side)
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-protection from damage
-absorbs water so helps with movement -important in cell recognition and adhesion |
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Transmembrane glycoprotein
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Glycosylation
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Glycosylation begins in ER
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Where does glycosylation begin?
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ER
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Asparagine
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Asn or N
lipid-linked oligosaccharide |
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Protein transferase
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transfers lipid-linked sugar from membrane/lipid link to growing polypeptide chain
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dolichol
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lipid that links oligosaccharide to membrane
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oligosaccharide
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many sugars
All proteins that will be glycosylated start in ER and get specific sugar chain, later modified in Golgi apparatus Typically added while protein is growing/being made |
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N-linked
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Nitrogen on asparigine (post modification)
Only glycosylated form is Asn + AA + serine/threonine |
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O-linked
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Oxygen on serine or threonine (post modification)
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