Cell Bio Lecture 38

Front 1

HPV can affect ___ which is in the ___ phase

Back 1

human papilloma virus can affect Rb which is in M phase

Front 2

HPV is a very diverse and species specific group of viruses

Back 2

-100 different types have been identified
-30-40 hpv types affect the anogenital tract, of these 15-20 are high-risk types associated with cancer, and 10-15

Front 3

hpv
high risk and low risk most common

Back 3

high risk 16 and 18
6 and 11 are low risk

Front 4

any sickness is a simple

Back 4

fact of the cell going wrong

Front 5

assembly of HPV particle

Back 5

360 copies of L1 protein
72 capsomers (pentamer of 5 L1s)
+ virol DNA + L2 protein

Front 6

assembly of HPV particle explained

Back 6

5 L1 monomers asssemble into a pentamer or capsomer, the basic structural unit of the virus particle. Then 72 capsomers assemble with L2 protein and viral DNA to form the virus particle

Front 7

the basic structural unit of the virus particle is

Back 7

a pentamer or capsomer

Front 8

important functions of viral proteins

Back 8

-e6:
-e7
L1
L2

Front 9

e6

Back 9

destruction of the p53 tumor suppressor protein

Front 10

e7

Back 10

inactivation of the Rb tumor suppressor protein

Front 11

L1

Back 11

forms VLP (viral protein coat)

Front 12

L2

Back 12

binds to DNA

Front 13

dysplasia

Back 13

alteration in shape, size, and organization of cells
-different from neoplasia which refers to abnormal cell growth

Front 14

HPV binds and infects teh ____

Back 14

basal cells in the basal layer of the epithelium

Front 15

CIN- cervical intraepithelial neoplasia

Back 15

classifies severity of dysplasia, in a cervical tissue specimen

Front 16

the important cell bio of HPV and cellular consequences of infection

Back 16

1) hpv e6 & e7 are produced as basal cells divide and move towards the outside of the mucosa & become the PB & SQ layer of the mucosal epithelium

Front 17

2 once here, e6 & 7 inhibit p53 & rb protein which normally prevent

Back 17

cell division in these layers. There fore the cell are free to divide and produce the thickened skin which is characteristic of a genital wart

Front 18

3 dna replicates as an episome in the ___

Back 18

PB and squamous layer. also in these layers, L1 & L2 are produced

Front 19

4 the significance of this cycle is that hpv delays production of immunogenic L1 and L2 until the

Back 19

cells have terminally differentiated into squamous epithelium which is sloughed off and not accessible to immune cells

Front 20

genital wart is a

Back 20

disregulation in cell cycle

Front 21

5 thus an immune response to L1 & L2 proteins is

Back 21

slow to appear and doesn't occur in all individuals infected with HPV

Front 22

6 high levels of e6 & 7 (in Cin3) may lead to the development of

Back 22

cervical cancer

Front 23

lifetime risk for sexually active men and women is at least

Back 23

50%

Front 24

by 50 years of age, at least 80% of women will have accquired

Back 24

genital HPV infection

Front 25

estimated incidence
estimated prevalence

Back 25

6.2 mil/year
20 mil

Front 26

routes of HPV transmission

Back 26

sexual routes: sex, g-g, hand-g, oral-g
non-sexual: extragenital-fomitos
vertical: mother-neonate-respiratory papillomatosis

Front 27

risk factors for HPV infection
females
males

Back 27

females: 20-24, young age of sex, # partners, uncircumcised male
males: 25-29, # partner, being unc

Front 28

key point: hpv 6 and 11 are responsible for

Back 28

>90% of anogenital warts

Front 29

indications for treatment

Back 29

bothersome symptoms: pruritus, bleeding, burning, discharge, pain
-obstruction of the vagina
-treatment is not mandatory as 20-30% spontaneously regress

Front 30

treatment modalities

Back 30

chemical and physical destruction
-surgical excision
-applied topical solution/drugs

Front 31

vaccines

Back 31

an antigenic substance administered to produce immunity to a disease thereby preventing the effects of infection

Front 32

18-25 yr old women: 1 partner
2 partner
3 partner

Back 32

14.3 hpv
22
32%

Front 33

fomites:

Back 33

anything that you can spread outside the bodies (you have to have the secretions on it) low risk

Front 34

what percent of cervical cancer is from hpv?

Back 34

100%

Front 35

types of vaccines

Back 35

inactivated vaccine
attenuated vaccine
subunit/recombinant vaccine

Front 36

inactivated vaccine

Back 36

-consists of virus particle grown in culture then killed
-particles are unable to replicate
-capsid proteins "survive" and illicit an immune response
-boosters are required periodically
-hep A

Front 37

attenuated vaccine

Back 37

live virus particles are administered
-particles are of low virulence
-particles are able to reproduce but very slowly
-boosters are required infrequently
-MMR, Varivax

Front 38

subunit/recombinant vaccine

Back 38

2 methods
1) placement of a protein gene from the targeted virus into another virus. the second virus expresses the protein, but provides no risk to the patient
2) administration of an isolated protein virus

Front 39

gardasil summary

Back 39

addresses HPV types associated with cervical dysplasias and genital warts
-induces amnestic response
-vaccination is prophylactic and max. efficacy is with those w/o previous exposure to HPV

Front 40

gardasil

Back 40

4 capsids- tetravalent
6,11,16,18
6,11 related to 90% of cc
16,18 70% cc