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56 Cards in this Set
- Front
- Back
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The ability of the microscopt to separate small details is defined as...
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resolution
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Sections 90nm thich are commonly cut with a/an...
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ultramicrotome
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Tissues embedded in glycol methacrylate are commonly cut with a...
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rotary microtome
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When cutting sections from paraffin blocks, the mose common cause of unsatisfactory sections is...
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a dull or improperly sharpened knife
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The regular laboratory incubator maintains a temperature of...
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37°C
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When the magnification can be changed without the need to refocus, the microscope objectives are said to be...
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parfocal
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If a ribbon splits when cutting paraffin sections, the trouble is most likely due to...
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nicks in the knife
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The bevel angle of most American manufactured knives is...
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28° to 32°
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The temperature of the oven used to maintain a supply of melted paraffin for embedding tissue should be about...
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60°C
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Doubly refractile particles are examined using...
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polorized light
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Crooked paraffin ribbons may be caused by...
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nonparallel block horizontal edges
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When using a microscope with a 10x ocular and a 40x objective, the total magnification is approximately...
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400
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The clearance angle of the microtome knife should be...
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3° to 8°
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The ordinary refrigerator, when operating normaly, has a temperature that approaches...
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4°C
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When a lens for a light microscope has been corrected for two colors, it is said to be...
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achromatic
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When using paraffin with a melting point of 55°C to 57°C, the mose common temperature for floating sections on a flotation bath is approximately...
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45°C to 50°C
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Compressed or wrinkled sections may be caused by...
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too little knife tilt
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The scanning objectives on the light microscope is found...
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at the lower end of the body tube
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Various sized holes are noted in sections of paraffin-embedded liver on the flotation bath. With further ribboning, these holes decrease in size and disappear. The most likely cause of the holes is...
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facing the block too aggressively
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Birefringent substances are best examined with which of the following microscopes...
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polarizing
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During microtomy, the sections lift from the knife as the block is raised. The most likely cause is...
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a dull knife
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Microscopic examination of an H and E stained section reveals marked chatter, especially at the edges of the tissue. This was most likely caused by...
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overdehydration of the tissue
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During cryotomy, sections of carying thickness are obtained. This can most likely be corrected by...
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increasing the knife tilt
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When checking the pH of a staining solution, the pH meter should be calibrated using a standard solution with a pH value...
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closest to that of the staining solution
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The microwave oven creates heat in staining solutions by...
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nonionizing radiation
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Tissue components can be measured with the light microscope ina process known as...
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micrometry
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A microscope that has two eyepieces is...
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binocular
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When a lens for a light microscope has been corrected for three colors it is said to be...
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apochromatic
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The high dry objective on most microscopes has a magnification of approximately...
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45x
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Tissues are subjected to a series of different reagents in a closed processor by...
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fluid transfer
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When processed on a short cycle, tissue must be...
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cut thin during grossing
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What are the names of the types of microscopes?
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-electron
-polarizing -light -darkfield -fluorescent -phase contrast |
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What temperature should the laboratory incubator be kept at?
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37°C
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What temperature should the infiltrating paraffin container be kept at?
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60°C
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What temperature should the flotation bath be kept at?
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45°C
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What temperature should the cryostat be kept at?
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-20°C
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What temperature should the refrigerator be kept at?
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4°C
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What temperature should the freezer be kept at?
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-50°C to -70°C
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T/F: Living cells are usually examined with the dark field micrscope.
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false
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T/F: A three-dimensional image is obtained with the transmission electron microscope.
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false
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T/F: Another name for the ocular of a microscope is the eyepiece.
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true
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T/F: The analyzer of the polarizing microscope is placed between the specimen and the eye.
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true
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T/F: Steel knives are commonly used to section glycol methacrylate.
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false
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T/F: The angle formed by the block face and the cutting facet of the knife is known as the bevel angle.
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false
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T/F: When examined microscopically, 8um sections will show all nuclei in the same plane of focus.
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false
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T/F: Slow freezing of tissue leads to the formation of ice crystals.
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true
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T/F: An overheated cryostat motor is frequently caused by ice buildup in the cryostat chamber.
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false
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T/F: If the histotechnologist is skilled, then the most critical factor in the laboratory becomes the care and maintenance of laboratory instrumentation.
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true
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T/F: Routine care of the microtome requires a thorough cleaning and oiling every 2 weeks.
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false
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T/F: Dust collecting on the cooling coils of the cryostat may cause the motor to run more slowly.
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false
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T/F: The temperature of the room is very important when cutting frozen sections.
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false
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T/F: Paraffin sections will not adhere well to clean untreated glass slides.
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true
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T/F: Poly-L-lysine is a common additive to the flotation bath.
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false
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T/F: Flotation bath water is a possible source of contamination on sections to be stained for microorganisms.
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true
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T/F: It does not matter how the knife is placed in the knife holder when using a mechanical sharpener.
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false
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T/F: Ideally, a thick layer of abrasive is needed for sharpening knives on a mechanical sharpener.
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false
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